Abstract：The regulator of colanic acid capsule synthesis (Rcs) is a complicated two-component signal transduction system. Although details of Rcs in most Enterobacteria are known, its regulation in Shigella has not been reported yet. This study aims to explore the influences of environmental stresses on Rcs transcription in Shigella. Based on biological informatics analysis of RcsBDC in Shigella flexneri 2a 301, the transcriptional levels of rcsB, rcsD, rcsC at different growth stages were analyzed by real-time polymerase chain reaction (PCR), and the changes in the transcriptional levels of the three genes in acid and osmotic pressure conditions were examined. The transcriptional levels of the three genes were higher in 5－6 h (logarithmic phase), and lower in 8－10 h (stationary phase). The transcriptional levels of rcsB and rcsD in acid and osmotic pressure conditions were higher than in normal condition, suggesting environmental stimuli promote the transcriptional levels of rcsB and rcsD in Shigella flexneri during stationary phase.
叶立娜，蔡霞，林志伟，吕智慧，王小飞，尚永朋，瞿涤. 外环境压力对福氏志贺菌荚膜异多糖酸合成调节子转录的影响[J]. 微生物与感染, 2018, 13(3): 155-164.
YE Li-Na, CAI Xia, LIN Zhiwei, LV Zhihui, WANG Xiaofei, SHANG Yongpeng, QU Di. Impact of environmental stresses on transcriptional regulation of Rcs in Shigella flexneri. JOURNAL OF MICROBES AND INFECTIONS, 2018, 13(3): 155-164.