Impacts of Mycobacterium tuberculosis RpsI on ribosomal structure and function in Mycobacterium smegmatis
SU Meng, LÜ Liangdong
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China
Abstract:Recent studies demonstrate that the structure of ribosome was committed to dynamic regulation during cell development and stress adaptation. We used NCBI BLAST to analyze the sequence similarity of ribosomal protein RpsI, RpmI and RpmJ between Mycobacterium tuberculosis and Mycobacterium smegmatis and found a striking variation in RpsI N terminal. To further study the influence of this variation on ribosomal function, we constructed a M. smegmatis recombinant strain in which the original rpsI was replaced by the counterpart from M. tuberculosis. The genotype of this strain was verified by PCR. We estimated the growth rate of recombinant strain and empty-control strain under different temperatures. Universal serial liquid dilution method was applied to measure the minimum inhibition concentration (MIC90) of five different ribosome-targeting antibiotics. We found that the recombinant strain became more sensitive to amikacin which binds to the A site of ribosome. These results demonstrate that the sequence variation in Mycobacterial RpsI may impact the structure of ribosomal A site.
苏梦,吕亮东. 分枝杆菌RpsI序列差异对核糖体结构与功能的影响[J]. 微生物与感染, 2020, 15(5): 293-301.
SU Meng, Lü Liangdong. Impacts of Mycobacterium tuberculosis RpsI on ribosomal structure and function in Mycobacterium smegmatis. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(5): 293-301.