In vitro antimicrobial activity of tetracycline against clinical isolates of Streptococcus agalactiae
ZHAO Linshan, XU Guangjian, CHEN Junwen, SUN Xiang, DENG Qiwen, YU Zhijian, LI Duoyun
Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infections, Nanshan People’s Hospital, Shenzhen 518052, Guangdong Province, China
Abstract:The present paper aims to investigate the antimicrobial activity of tetracycline against clinical Streptococcusagalactiae (S. agalactiae) isolates. A total of 136 S. agalactiae isolates were collected retrospectively in Nanshan People’s Hospital between 2014 and 2017. The minimum inhibitory concentrations (MICs) of tetracycline were determined by agar dilution. Polymerase chain reaction (PCR) was used to detect Tet-specific resistance factors and housekeeping genes. The strains were typed by multilocus sequence typing (MLST). The results showed that the resistance rate of S. agalactiae isolates to tetracycline was 46.32% (63/136), and the tetracycline-resistant strains mainly carried tetM. The dominant ST types were ST17 and ST19 strains. The antimicrobial activity of tetracycline against ST17 strains was significantly higher than ST19 strains. It is concluded that the tetracycline resistance in S. agalactiae isolates is associated with tetM gene and the dominant strains are ST17 strains and ST19 strains.
赵琳珊,徐广健,陈俊文,孙翔,邓启文,余治健,李多云. 无乳链球菌临床株对四环素类的敏感性研究[J]. 微生物与感染, 2021, 16(1): 45-49.
ZHAO Linshan, XU Guangjian, CHEN Junwen, SUN Xiang, DENG Qiwen, YU Zhijian, LI Duoyun. In vitro antimicrobial activity of tetracycline against clinical isolates of Streptococcus agalactiae. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(1): 45-49.