Th2 immunity to HBsAg primed by gene gun DNA vaccination can be shifted towards Th1 immunity by co-delivery or co-expression of HBV core gene
ZHOU Xiao-hui; LIU Ling; LIU Hua; XIANG Li; YUAN Zheng-hong
Key Laboratory of Medical Molecular Virology of Ministry of Education and Public Health of China, Shanghai Medical School, Fudan University, Shanghai 200032, China
Objective To investigate the potential of co-expression or co-delivery of HBV core gene with plasmid DNA to shift HBsAg-specific Th2 immune response induced by gene-gun to Thl type.Methods pIRES/Core, pIRES/C 149,pIRES/S,pIRES/S/Core and pIRES/S/C149 were constructed and expression of recombinant plasmids were confirmed in eukaryotic cells. BALB/c mice were immunized by gene-gun. After being boosted twice, humoral and cellular immunities in immunized mice were monitored. Results HBV core gene enhanced the HBsAg-specific Thl type immune responses in gene-gun-DNA-immunized BALB/c mice, including increasing the liter of IgG2a, ratio of IgG2a/IgG1,CTL activity as well as IFN-γsecretion. Conclusion Co-delivery or co-expression of HBV core gene with HBsAg DNA vaccine by gene gun can shift the HBsAg-specific Th2 immune response to Th1 type.
周晓辉; 刘玲; 刘华; 项黎; 袁正宏. 基因枪接种乙型肝炎表面抗原促进DNA疫苗诱生的免疫应答转换[J]. 微生物与感染
, 2006, 1(1): 15-20 .
ZHOU Xiao-hui; LIU Ling; LIU Hua; XIANG Li; YUAN Zheng-hong. Th2 immunity to HBsAg primed by gene gun DNA vaccination can be shifted towards Th1 immunity by co-delivery or co-expression of HBV core gene
. Journal of Microbes and Infections, 2006, 1(1): 15-20 .
