Abstract:Objective To develop a rapid and sensitive assay system to detect the seven virulence factors of Streptococcus suis,using two seqarate multiplex PCR assays.Methods The seven virulence factors were detected based on the gene sequences of mrp,epf,sly gdh,gapdh,orf2 and fbps.PCR primer pairs were produced for each of the 7 factors and the assay method,was developed and optimized.These genes were tested in 29 bacterial isolates by the multiplex assay.Results All PCR products were analyzed by electrophoresis on 2% agarose gels.Detection of all 29 Streptococcus suis strains was confirmed by bacteriological examination.The assay was validated by the positive and negative controls.Conclusion The results demonstrate that the multiplex PCR assay is a highly specific and sensitive diagnostic tool for the detection of seven virulence factors of Streptococcus suis. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
李小军; 张苏华; 刘佩红; 王建; 沈莉萍; 姚火春; 陆承平. 多重聚合酶链反应检测猪链球菌7种主要毒力因子[J]. 微生物与感染
, 2007, 2(1): 30-33 .
LI Xiao-jun; ZHANG Su-hua; LIU Pei-hong; WANG Jian; SHEN Li-ping; YAO Huo-chun; LU Cheng-ping. Detection of seven virulence factors of Streptococcus suis by multiplex PCR assay. Journal of Microbes and Infections, 2007, 2(1): 30-33 .