Viperin protein inhibits hepatitisC virus replication partially through
disturbing the association of nonstructural proteinswith lipid rafts
WU Xian-Fang1; WANG Shan-Shan1; PAN Ting-Ting2; YI Zhi-Gang2; YUAN Zheng-Hong1,2
1. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China；2. Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical School, Fudan University, Shanghai 200032, China
Abstract：Infection with hepatitis C virus (HCV) is currently treated with interferon-α( IFN-α) , but the molecular mechanismby which IFN inhibits HCV replication still remains elusive. Therefore, in this study, we attempted to elucidate the possible mechanismfor the IFN-inducible protein, Viperin, against HCV. Over-expression of Viper in could significantly down-regulate intracellular levels of HCV protein and RNA in both the replicon and HCVcc systems. Membrane flotation analysis indicated that expression of Viperin could interfere with the association of HCV NS3 and NS5A with the lipid raft, resulting in increased sensitivity of these two proteins to the treatment of non-ionic detergent. Furthermore, Viperinwas able to interact with FPPS because co-transfection of Viperin with FPPS could partially reverse the anti-HCV effects of Viperin in replicon cells. The above results indicate a possible novel mechanismof IFN-induced antiviral activity, that is, over-expression of Viperin can interfere with the microdomain of intracellular lipid raft and, therefore, further disrupt the stability of viral replicase complex to inhibit its functions.
吴显芳1; 王珊珊1; 潘婷婷2; 易志刚2; 袁正宏1, 2 . Viperin蛋白可通过影响非结构蛋白与脂筏的关联抑制丙型肝炎病毒复制[J]. 微生物与感染
, 2009, 4(4): 203-208 .
WU Xian-Fang1; WANG Shan-Shan1; PAN Ting-Ting2; YI Zhi-Gang2; YUAN Zheng-Hong1, . Viperin protein inhibits hepatitisC virus replication partially through
disturbing the association of nonstructural proteinswith lipid rafts. Journal of Microbes and Infections, 2009, 4(4): 203-208 .
2009, Vol. 4 
