Technical advances in laboratory diagnosis of fungal infection
Qinfang Qian; James E. Kirby
Division of Laboratory and Transfusion Medicine, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA02215, USA
Abstract：Rapid detection and identification of fungi from clinical specimens and culture isolates has become increasingly important as the numbers of patients susceptible to these infections continues to grow. Fortunately, new assays have been recently developed to aid early diagnosis and guide appropriate empiric anti-fungal therapy. Major improvement has been focused on direct detection of fungal antigen in specimens (e.g., galactomannan and beta glucan); rapid culture-based identification methods including chromogenic Candida agar; automated biochemical test panels such as Vitek 2 and MicroScan; and molecular assays including peptide nucleic acid fluorescent in situ hybridization (PNA-FISH), specific and broad range polymerase chain reaction(PCR) assays, and DNA-based sequencing technology use to identify organisms directly in clinical specimens or from positive cultures.
钱琴芳; James E. Kirby . 真菌感染的实验室诊断研究进展[J]. 微生物与感染
, 2010, 5(1): 2-25 .
Qinfang Qian; James E. Kirby. Technical advances in laboratory diagnosis of fungal infection. Journal of Microbes and Infections, 2010, 5(1): 2-25 .
2010, Vol. 5 
