Enhancement of the mucosal immune responses and prophylactic effect of a novel coxsackievirus B3-specific mucosal DNA vaccine by co-administration of lymphotactin
Yue Yan; Hu Lin-Kun; Xu Wei; Xiong Si-Dong
Institute for Immunobiology, Shanghai Medical college, Fudan University, Shanghai 200032, China
Abstract：To improve the mucosal immune responses and protection against coxsackievirus B3 (CVB3), on the basis of our previously prepared chitosan-pVP1 mucosal vaccine, plasmid encoding lymphotactin (LTN), a C family chemokine, was introduced. chi-(pVP1+pLTN) were prepared by conjugating chitosan with the mixture of 50 μg pVP1 and pLTN. Male BALB/c mice were immunized 4 times biweekly. Two weeks after the final immunization, the level of CVB3-specific serum IgG, fecal IgA and mucosal CTL activity were quantified. Meanwhile, mice were challenged with 3 LD50s, serum creatine kinase (CK) activity and histopathological changes in heart tissue were measured. Compared with control groups, immunization with chi-(pVP1+pLTN) significantly increased the levels of CVB3-specific serum IgG and fecal IgA, and enhanced the mucosal CTL activity. The incidence of myocarditis in mice receiving chi-(pVP1+pLTN) was 16.7%, significantly lower than 33.3% in mice receiving chi-(pVP1+pcDNA3.1). Histopathological analysis showed that fewer inflammatory cells under the epicardium in the hearts of co-immunized mice compared with that of control groups. In conclusion, intranasal co-administration of LTN plasmid via chitosan nanoparticle could enhance CVB3 specific mucosal immune responses and provide more efficient protection against CVB3-induced myocarditis.
岳艳; 胡林昆; 徐薇; 熊思东. 淋巴细胞趋化因子增强柯萨奇病毒DNA疫苗黏膜免疫及预防小鼠心肌炎的作用[J]. 微生物与感染
, 2010, 5(2): 77-83.
Yue Yan; Hu Lin-Kun; Xu Wei; Xiong Si-Dong. Enhancement of the mucosal immune responses and prophylactic effect of a novel coxsackievirus B3-specific mucosal DNA vaccine by co-administration of lymphotactin. Journal of Microbes and Infections, 2010, 5(2): 77-83.
2010, Vol. 5 
