Serotypes and virulence gene distribution among Vibrio parahaemolyticus in Shanghai, 2012
CHEN Hong-You1, SHENG Yue-Ying2, TU Li-Hong1, SONG Yuan-Jun1, ZHANG Xi1, CHEN Min1
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China; 2. Shanghai Institute of Health Sciences of Technology, Shanghai 201318, China
Abstract：The main purpose of the research is to find the most common serotypes and tdh and (or) trh carrying rate of Vibrio parahaemolyticus (V. parahaemolyticus) isolates from diarrhea cases and food in Shanghai, 2012. Isolates were collected in diarrhea surveillance system from 15 district Centers for Disease Control and Prevention (CDCs). All isolates were serotyped with O and K antisera. tdh and trh genes were detected by a duplex polymerase chain reaction (PCR) assay. Totally 88.1% of all 854 isolates could be serotyped with both O and K antisera, and 89.8% were virulent. Among the serotyped isolates from diarrhea cases, 93.8% were O3:K6 (56.2%), O4:K8 (13.5%), O1:K25 (6.8%), O4:K68 (7.0%), O4:K9 (4.5%), O1:K36 (2.9%) and O3:K29 (2.9%), which were considered as the most common serotypes in Shanghai. Central tendency was shown in the distribution of all isolates by month, and the peak appeared during July to August. While for isolates of O4:K9 and O1:K36 serotypes were different without central tendency in monthly distribution. The serotypes distribution in food isolates was different from that of diarrhea isolates. The proportion of virulent strains in diarrhea isolates (95.6%) was higher than that in food isolates (5.5%). Among diarrhea isolates, the proportion of virulent strains in the most common serotypes (99.9%) was higher than that in non-common serotypes (71.1%). tdh detection rate in the serotypeable (97.5%) was higher than that in the untypeable (67.6%), but trh detection rate in the serotypeable (0.8%) was lower than that in the untypeable (42.6%). The results hint that the serotype constitute is different from that of historical data, and tdh（trh）carrying or not is likely to be related to serotypes. The isolates from diarrhea cases and food show separation phenomena in serotypes and virulent factors, indicating the importance of serotyping and virulent factor detection in surveillance and detection of V. parahaemolyticus.
陈洪友1,盛跃颖2,屠丽红1,宋元君1,张曦1,陈敏1. 2012年上海地区副溶血性弧菌血清分型和毒力基因携带状况研究[J]. 微生物与感染, 2014, 9(1): 37-42.
CHEN Hong-You1, SHENG Yue-Ying2, TU Li-Hong1, SONG Yuan-Jun1, ZHANG Xi1, CHEN Min1. Serotypes and virulence gene distribution among Vibrio parahaemolyticus in Shanghai, 2012. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(1): 37-42.
