Abstract：This study aims to investigate the epidemiology of human cytomegalovirus (HCMV) among pregnant women and hospitalized children in central China area, and to evaluate the value of different samples 〔breast milk, urine and peripheral blood mononuclear cells (PBMCs)〕 for diagnosis of HCMV infection. HCMVspecific IgM and IgG antibodies were determined by electrochemiluminescence immunoassay to verify the infection status of HCMV. DNA isolated from different samples was subjected for the detection of HCMV specific gene(s) by realtime fluorescence quantitative polymerase chain reaction (PCR). For pregnant women and hospitalized children, the positive rate of IgM was 1.16% and 8.13% respectively. The rates for newborns and preterm infants were 2.06% and 3.50%. The positive rates of IgG for HCMV among pregnant women and hospitalized children were 96.59% and 79.98%, respectively, while the rates among newborns and preterm infants were 83.84% and 94.59%, respectively. The positive rates of HCMV DNA detection were 57.25% and 7.97% for urine and PBMCs, respectively (P<0.001). 44.61% of mothers shed HCMV DNA in breast milk, while 0.77% were found positive in PBMCs (P<0.001). The results suggest that HCMV infection rate in pregnant women is considerably high and HCMV is one of the pathogens for hospitalized children infection. The preterm infants seem more sensitive to HCMV infection than the term infants, and the analysis of the presence of viral DNA from urine in hospitalized children is more suitable for the diagnosis of HCMV infection. HCMV DNA has a higher positive rate in breast milk than in PBMCs from lactating mothers and maternal milk is the main source for HCMV transmission from mother to infant.
王婷，段歌红，陈茂才，冀恒涛，崔会玲. 人巨细胞病毒在华中地区孕妇和住院儿童群体中的流行病学调查[J]. 微生物与感染, 2015, 10(5): 288-293.
WANG Ting, DUAN Ge-Hong, CHEN Mao-Cai, JI Heng-Tao, CUI Hui-Ling. The epidemiology of human cytomegalovirus among pregnant women and children in central China area. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(5): 288-293.
