Biological characterization and genome sequence of KP002, a novel bacteriophage isolated from multiple-drug resistant Klebsiella pneumonia
GAO Jing12, XU Bin3, GUO Xiaokui1, QIN Jinhong1
1. Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2. Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China; 3. Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Chongming Branch, Shanghai 202150, China
Abstract：Multiple-drug resistant Klebsiella pneumonia strains collected from several hospitals in Shanghai were used as the host bacteria to isolate phages in the wastewater. A novel phage KP002 was isolated and analyzed in details. The morphology and size of the bacteriophage was observed by electron microscope with negative staining. The results indicated that it was a tailed phage with head diameter about 70 nm, tail length about 80 nm and tail width about 20 nm. This phage had high activity at pH from 3 to 9 and temperature from 4 to 50 ℃. The adsorption rate was above 95% within 6 min. Its incubation period was 10 min and burst period was 50 min. The burst size reached 172 pfu/cell. The whole genome sequence showed that it contained a circular double-stranded DNA with a fulllength of 47 173 bp and GC content of 48%. KP002 is a new phage against drug-resistant bacteria and could be served as a potential resource for clinical treatment of multiple-drug resistant bacterial infection.
