In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases
ZHAO Keqing1, YANG Chen1,2, WU Yang3, GUO Limin1, LIU Huayong3, JI Jian4, CHEN Xiaoying5, ZHENG Chunquan1
1. Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University; Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai 200031, China; 2. Department of Otolaryngology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine,, Shanghai 200035, China; 3. Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; 4 . Department of Clinical Laboratory, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China; 5. Department of Dermatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200035, China
Abstract：In photodynamic therapy (PDT), the reactive oxygen species generated by the activation of photosensitizers by light of an appropriate wavelength, are able to inactivate microbes by damaging various microbial components. Due to its multi-targeting antimicrobial capability, PDT has shown a potential as an effective alternative therapeutic option against methicillin-resistant Staphylococcus aureus (MRSA). In this study, MRSA strains isolated from burn, acute pharyngitis, nasosinusitis and pneumonia patients were subjected to a 5-aminolevulinic acid (ALA) mediated light emitting diode (LED) array in vitro. The results showed that a significant reduction (1.8 log10 cfu) was achieved with an ALA treatment (1 h, 5 mmol/L) followed by 1 h of illumination [(633±10) nm wavelength light source, and a 360 J/cm2 light dose]. MRSA strains collected from different tissues showed a similar sensitivity to the treatment.
赵可庆1，杨晨1.2，吴旸3，郭丽敏1，刘华勇3，吉建4，陈小英5，郑春泉1. 5-氨基酮戊酸光动力疗法对不同疾病来源的耐甲氧西林金黄色葡萄球菌的体外杀伤作用[J]. 微生物与感染, 2016, 11(4): 228-233.
ZHAO Keqing1, YANG Chen1.2, WU Yang3, GUO Limin1, LIU Huayong3, JI Jian4, CHEN Xiaoying5, ZHENG Chunquan1. In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(4): 228-233.
2016, Vol. 11 
