Subculture of chicken embryo cells for propagation of measles virus
ZHANG Linya1, XU Ran1, ZHOU Xu2, YANG Wenzhen2, ZHU Wei1
1. The Third Laboratory of Shanghai Institute of Biological Products Co., Ltd., Shanghai 200051, China; 2. Shanghai Institute of Biological Products Co., Ltd., Shanghai 201400, China
Abstract：To establish the subculture technology of primary chicken embryo cells and explore the sensitivity and adaptability of passage chicken embryo cells to measles virus, the primary chicken embryo cells were subcultured, and the measles virus Shanghai-191 (S-191) strain was cultured in primary chicken embryo cells and subcultured chicken embryo cells. The titer of the virus harvest liquid was detected and the gene sequence was determined. The results showed that the primary chicken embryo cells could be stably subcultured to the 10th generation, and the growth trend of each generation was similar. The chromosome examination of the 5th generation chicken embryo cells showed normal chromosome karyotype, and the tumorigenicity test of the 8th generation chicken embryo cells showed no tumorigenesis. The titers of measles virus prepared from the 3rd and 5th passage of chicken embryo cells were higher than that of the primary chicken embryo cells, but there was no significant difference (n＝3, P＞0.05). The gene sequences of the nucleoprotein(N)and hemagglutinin(H)of the virus were completely consistent with those of S-191 strain, and there was no variation. This study confirms that the primary chicken embryo cells can be subcultured, and the sensitivity of each generation of chicken embryo cells to measles virus remains unchanged. There is no significant difference in the level of measles virus production, which could be used to culture measles virus.
2022, Vol. 17 
