Investigation of mosquitoes and mosquito-borne arboviruses in Gansu Province,China
YU Deshan1,*, CUI Shiheng2,*, FU Shihong3,*, CAO Lei3, CAO Yuxi3, CHEN Shenbang1, LI Guotai1, JIANG Jianxiang1, LIANG Guodong3
1. Gansu Provincial Center for Disease Control and Prevention, Gansu 730000, China; 2. Qingdao University, Qingdao 266071, China; 3. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract:The purpose of the current study is to investigate the distribution of mosquitoes and mosquito-borne arboviruses in Gansu Province. The mosquitoes were collected from Jiuquan and Longnan in 2014. 2 833 mosquitoes were collected from Jiuquan, including Aedes dorsalis 2 665 (94.1%), Aedes vexans 150 (5.3%) and Culex pipiens pallens 18 (0.6%). 1 579 mosquitoes were collected from Longnan, including Culex pipiens pallens 1 451 (91.9%) and Anopheles sinensis 128 (8.1%). All mosquitoes were divided into 96 pools and then inoculated onto C6/36, BHK and Vero cells for virus isolation and identification. The results showed that the 96 pools of mosquitoes inoculated on three cells revealed negative cytopathic effect (CPE). Eighteen pools of the mosquito samples showed positive for Liaoning virus (LNV) detection. The genetic evolution analysis of viral nucleotide sequences showed that Liaoning virus in Gansu Province had the closest relationship with the Liaoning virus isolated in Xinjiang Uygur Autonomous Region.
于德山1,*,崔世恒2,*,付士红3,*,曹蕾3,曹玉玺3,陈生邦1,李国太1,蒋建祥1,梁国栋3. 2014年甘肃省部分地区蚊虫及蚊媒病毒调查[J]. 微生物与感染, 2016, 11(3): 153-158.
YU Deshan1,*, CUI Shiheng2,*, FU Shihong3,*, CAO Lei3, CAO Yuxi3, CHEN Shenbang1, LI Guotai1, JIANG Jianxiang1, LIANG Guodong3 . Investigation of mosquitoes and mosquito-borne arboviruses in Gansu Province,China. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(3): 153-158.