Abstract：The aim of the present study was to detect the Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization. The BDV p24 sense probe was labeled with digoxigenin ( DIG) using a DIG RNA labeling kit. The labeling efficiency of the BDV p24 sense probe was conformed by spot test, and the specificity was determined by a dot hybridization assay. The BDV genome was then detected with the probe via in situ hybridization in paraffin brain sections of BDV-inoculated Wistar rats. At the third week post-BDV inoculation, the BDV genome was detected mainly in hippocampus and cortex, with minimal labeling in the thalamus and hypothalamus. Whereas at the sixth week post-BDV inoculation, the BDV genome was not only detected in hippocampus and cortex, but was also increased in thalamus and hypothalamus, suggesting that the BDV-infected region was enlarged following prolonged post-inoculation time, and that in situ hybridization with a BDV p24 sense probe can be used to observe the distribution of BDV in the inoculated Wistar rat brain.
刘爱平；李玉军；李小光；宋武琦；李爱梅；周淑如；张凤民. 博尔纳病病毒接种Wistar 大鼠脑组织中病毒基因组的原位检测[J]. 微生物与感染
, 2009, 4(3): 152-155 .
LIU Ai-Ping；LI Yu-Jun；LI Xiao-Guang；SONG Wu-Qi；LI Ai-Mei；ZHOU Shu-Ru；ZHANG Feng-Min. Detection of Borna disease virus ( BDV) genome in the brain of BDV-inoculated Wistar rats by in situ hybridization. Journal of Microbes and Infections, 2009, 4(3): 152-155 .
