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| Detection of virulence capacity and genes for viable but non-culturable Campylobacter jejuni |
| YANG Jie-lin; DOU Tong-hai; GU Min; WU Zhong-liang |
| Shanghai Bureau Entry and Export Inspection and Quarantine,Shanghai 200135, China |
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Abstract Objective The existence of a viable but non-culturable(VBNC)state has been described for Campylobacter jejuni.This state can be induced and detected using established VeroE6,HeLa cell lines.Methods VBNC C.jejuni cell were induced by low temperature and starvation.Sensitization test of established HeLa,Vero cells model to CDT of viable but non-culturable Campylobacter jejuni showed that the cell was still sensitive,and the result was further confirmed by RT-PCR test.The experiment found that VBNC C.jejuni cell still had the virulence to cell cultures although it decreased.Total and active cells were counted by conventional culture method and after staining with 49,6 diamino-2-phenylindole(DAPI).Induced VBNC cell were obtained by freezed at-70 ℃.Results The rest VBNC cell were confirmed by different state cell count.C.jejuni decreased about one magnitude after refrigeration.About 70% cell can be induced into apotosis before refrigeration and only 40% cell die after C.jejuni refrigeration.Conclusion Low temperature and nutrient poor conditions can induce cell enter into VBNC state.Remarkable change of C.jejuni cell to produce virulence can be observed,and we confirmed this further at RNA level.VBNC C.jejuni cell can still produce virulence although the quantity decreased remarkably. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
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Received: 01 January 1900
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Corresponding Authors:
YANG Jie-lin
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2007, Vol. 2 
