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Efficacy and stability of maraviroc gel as a microbicide candidate in vitro |
LI Liang-Fen1, BEN Yin-Yin1, LI Liang-Zhu1, ZHANG Xiao-Yan1,2 |
1. Shanghai Public Health Clinical Center Affiliated to Fudan University,Institutes of Biomedical Sciences,Fudan University,Shanghai 201508, China; 2. Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Basic Medical Sciences, Fudan University,Shanghai 200433, China |
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Abstract To study the efficacy and stability of maraviroc gel in vitro, the cytotoxicity of maraviroc in phosphate buffered saline (PBS) or in 0.015% hydroxyethyl cellulose (HEC) were evaluated in TZM-bl and Jurkat-T cells. Meanwhile, two subtypes of human immunodeficiency virus (HIV) pseudovirus (SVPB16 and SVPC12) were used to evaluate the anti-HIV activity in vitro, the half maximal inhibitory concentration (IC50) or 90% maximal inhibitory concentration (IC90) of maraviroc gel were assessed. Furthermore, maraviroc was dissolved in 1.5% HEC with a final concentration of 6 mmol/L, and then maraviroc gel was stored at 4 ℃, room temperature, 30 ℃ and 40 ℃, respectively, in 75% relative humidity for 1 to 8 weeks. The anti-HIV activity was tested weekly. The data showed that maraviroc gel had nearly same cytotoxicity as maraviroc in PBS. HEC did not weaken the ant-HIV activity of maraviroc. Inversely, HEC might slightly enhance the anti-HIV efficacy of maraviroc. Maraviroc gel was stable for at least 8 weeks even at 40 ℃. It is concluded that maraviroc gel has good efficacy and stability as a microbicide candidate.
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Received: 18 February 2014
Published: 25 June 2014
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Corresponding Authors:
ZHANG Xiao-Yan
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