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| Effect of anti-YycG monoclonal antibody against Staphylococcus epidermidis biofilm formation |
| GONG Ting, HAN Hai-Yan, LV Zhi-Hui, WANG Xiao-Fei, CAO Yun, CHENG Xun-Jia, WU Yang, QU Di |
| Department of Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China |
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Abstract Bacterial YycFG two-component signal transduction system plays a major role in controlling cell wall synthesis, metabolism and biofilm formation of Staphylococcus. The extracellular PAS domain of YycG histidine kinase can sense various environmental signals. Therefore, a monoclonal antibody targeting PAS domain of Staphylococcus epidermidis (S. epidermidis) was produced (YycG-PAS MAb). Enzyme-linked immunosorbent assay (ELISA) indicated that YycG-PAS MAb belonged to IgG2a subclass and antibody titer was log 215. Western blotting suggested that YycG-PAS MAb had the ability to bind both recombinant YycG-PAS protein and protein extracted from cells. Further investigation exhibited inhibitory effect of YycG-PAS MAb (80 μg/ml) against biofilm formation after co-incubation with S. epidermidis and the inhibitory rate was 46.5%. The anti-biofilm activity of YycG-PAS MAb was improved when combined with low-concentration of vancomycin (1 μg/ml). The inhibitory rate was increased from 57.6% to 93.0% (P<0.01). No significant effects of YycG-PAS MAb on viability and growth of planktonic bacterial cells were observed. This study indicated that YycG-PAS MAb is a promising candidate antibody for biofilm-related infections.
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Received: 20 April 2015
Published: 25 June 2015
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Corresponding Authors:
Di Qu
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