|
|
Clinical evaluation of three methods in detection of Clostridium difficile in stool |
ZHANG Lihua, LI Zhen, JIANG Cen, WAN Yinglei, PENG Yibing |
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China |
|
|
Abstract This study aims to evaluate the clinical performance of three methods in the detection of Clostridium difficile (C. difficile) in stool samples. Clinical samples were subjected to enzymelinked fluorescence assay for C. difficile toxins A and B (CDAB), conventional bacterial cultivation using cycloserine-cefoxitin-fructose agar (CCFA) and cultivation by chromogenic C. difficile identification medium (chromID™), respectively. A combination of bacterial culture and amplification of the tcdB gene was selected as the standard reference method for the evaluation. A total of 164 clinical stool samples were collected, of which 58 had positive results by the reference method whilst the rest 106 were negative. The CDAB method showed a sensitivity, specificity, positive predictive value and negative predictive value of 51.7%, 95.3%, 85.7% and 78.3%, respectively, while the corresponding values of the CCFA method turned out to be 72.4%, 98.1%, 95.5% and 86.7%, respectively. The chromID™ method gave corresponding values of 94.8%, 92.5%, 87.3% and 97.0%, respectively. Among the three methods, chromID™ method had the best consistency with the reference method (Kappa=0.856). It is concluded that chromID™ is a simple and costeffective method for the detection of C. difficile in stool samples, which can present easytojudge results and has a preferable value in clinical application.
|
Received: 16 July 2015
Published: 25 February 2016
|
Corresponding Authors:
WAN Yinglei
|
|
|
|
|
|
|