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| miR-125b-5p regulates mycobacteria growth in host cells and in mice |
| HUANG Guixian1,2, LIANG Shanshan2, PENG Ying1,2, SHEN Hongbo2, WANG Feifei3, XU Junfa1 |
| 1. Department of Clinical Immunology, Institute of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, Guangdong Province, China; 2. Clinic and Research Center of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China; 3. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), and Department of Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China |
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Abstract MicroRNAs (miRNAs) play an important role in the regulation of the immune system. To demonstrate the role of miR-125b-5p in anti-TB immunity, we have infected host target lung alveolar A549, human macrophage THP-1, mice macrophage RAW264.7 and C57BL/6 mice, respectively. The q-PCR results demonstrated that miR-125b-5p is highly expressed in the tested conditions. The expression of miR-125b-5p in BCG infected mice lung was about 15 times of that in the control mice. The results verified that miR-125b-5p regulated mycobacteria growth in A549, THP-1 and RAW264.7 cells, respectively. Moreover, administration of miR-125b-5p inhibitors in BCG infected mice significantly reduced bacteria burden in lung. Thus, we concluded that miR-125b-5p may play a critical role on mycobacteria proliferation in vivo. The results provide useful information for our understanding of host immune defense and potential therapeutic interventions against this disease.
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Received: 08 October 2019
Published: 25 April 2020
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Corresponding Authors:
XU Junfa，WANG Feifei
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2020, Vol. 15 
