WANG Le-yao; ZHONG Peng; SHAN Liang; ZHONG Jiang
Experimental study on the co-application of Rta-expressing baculovirus vector and ganciclovir for the therapy of nasopharyngeal carcinoma
Objective To test a novel baculovirus expressing Epstein-Barr virus (EBV) lytic replication immediate early gene Rta, in combination with antiviral chemical ganciclovir, for the activation of latent EBV in nasopharyngeal carcinoma (NPC) cells, and the killing of tumor cells. Methods A baculovirus vector with EBV replication origin OriP, and the Rta expression cassette led by CMV promoter, was used to treat EBV-latently infected NPC cell line Hone1-EBV, as well as nude mice tumor model established from the cell line, in combination with ganciclovir. The effect on the growth of cell and tumor was studied. Results When treated with recombinant baculovirus in combinantion with ganciclovir, the growth of EBV-positive cells was significantly reduced to only 51% of the control. In the nude mice experiments, tumor growth was also greatly reduced. At 10th day after the first injection, the average volume and weight of the treated group was only about 20% and 30% of that of the control group, respectively. Histological analysis indicated there was significant cell necrosis in the baculovirus-ganciclovir-treated tumor tissue. Conclusion Co-application of RTA-expressing baculovirus vector and gancyclovir may be useful for the therapy of EBV associated NPC.
LI Xiao-xiao; MA Zhang-mei; WEN Yu-mei
Studies on hepatitis B virus genotypes and responses to antigen-antibody complex therapeutic vaccine
Objective To study whether there are differences in the responses of chronic hepatitis B patients infected with different hepatitis B virus (HBV) genotypes to HBsAg-HBIG therapeutic vaccine. Method Serum samples from sixty seven chronic hepatitis B patients who completed the full course of immunization with 60?g HBsAg-HBIG (YIC) were assayed for their virus genotypes by PCR-RFLP, in association with their viral and HBeAg sero-conversion responses to YIC treatment. Results Among the 67 patients, 22 were infected with HBV genotype B, and 43 were infected with genotype C, while in 2 patients the genotype of HBV could not be identified. In association with the responses to YIC treatment, no significant therapeutic differences were found between patients who were infected with HBV genotype B or with HBV genotype C. Conclusion Infections with HBV genotypes B or C do not affect the therapeutic responses in chronic hepatitis B patients to YIC.
LOU Qiang; ZHU Tao; Wang Jia-xue; WU Yang; Zhu Yu-li; QU Di
Methods of constructing Staphylococcus epidermidis gene mutants via different plasmids
WANG Fang; YAO Kun; FENG Dong-ju; ZHOU Feng; YIN Quan-zhang
Establishment of HHV-6-specific CD4+ T cell clones
Objective To establish the HHV-6-specific CD4+ T cell clones and further study the character of HHV-6-specific CD4+ T cells. Methods We acquired CD4+ T cell clones by the liquid microwell limiting dilution technique, and selected HHV-6-specific CD4+ T cell clones by means of 3H-thymidine uptake. The phenotype of HHV-6-specific T cell clones were analyzed with FACS. We tested the IL-10, IL-4 or IFN-γ secretion from HHV-6-specific CD4+ T cell clones by ELISA. Results Of the five HHV-6-specific CD4+ T cell clones, four showed proliferative responses to the stimulation with HHV-6 infected JJHAN lysates. Moreover, proliferation responses of HHV-6-specific CD4+T cell clones depended on the concentration of the HHV-6-infected JJHAN lysates. The phenotypic analysis with FACS demonstrated that HHV-6-specific T cell clones consisted of CD3+ CD4+ T cells. CD4+ T cell clones-W-2 and W-4 possessed the feature of high levels of IL-10 production, while CD4+ T cell clones-W-1 possessed the feature of high levels of IL-10 and IFN-γ production and W-3 possessed the feature of high levels of IFN-γ production. Conclusions The HHV-6-specific CD4+ T cell clones, were established in our study so as to further study their pneotype, the character of cytokine secrection, and to provide experimental basis for selecting HHV-6-specific CD4+ Treg cell clones.
Li Xiang-dong; Shen Yang; Qiu Ya-feng; Ma Zhi-yong
Meq protein of Marek’s disease virus inhibits the transactivity of p53
Objective To seek better diagnostic tests for tuberculous pleural effusion. Methods Adenosine deaminase (ADA) and anti-TB antibody in pleural effusion were tested by modified Martineck’s method and ELISA in 50 cases of tuberculous pleural effusion, 45 cases of malignant pleural effusion and 30 cases of right cardiac insufficiency pleural effusion, respectively. Result The level of ADA and anti-TB antibody in pleural effusion remarkably higher in tuberculous pleural effusion than in malignant pleural effusion and right cardiac insufficiency pleural effusion (p<0.01). When ADA >45U/L and positive anti-TB antibody were present,their sensitivity, specificity and accuracy in diagnosis of tuberculous pleural effusion are 100%, 98.6%, 99.2% and 90% , 93.3%, 92%, respectively. Conclusion The tests of ADA and anti-TB antibody in pleural effusion are very important in diagnosis tuberculous pleural effusion.
CAI Xiao-di; CAO Yun; CHEN Chao; QIAN Tian; WANG Chuan-qing
Acinetobacter baumannii from ventilator-associated pneumonia in the NICU
LI Dan; LU Hong-zhou
New nomenclature for Pityrosporon ovale
HUANG Chong; ZHANG Xin-xin
Research progress in hepatocacinoma secondary to hepatitis C virus infection
FEN Yan; GUO Xiao-kui
Helicobacter pylori and gastric cancer
CHEN Jing-xian; JIN Qi
Ebola virus
LI Wei-zhong; LI Qi-han
Herpes simplex virus type I protein ICP0: an important molecule regulating the interactions between the virus and host cells
YANG Yang; GUO Xiao-kui
Research progress of bacterial pore-forming cytotoxin
XIAN Mo; WU Zhong-dao
Involvement of innate immunity in anti-colonization caused by Streptococcus pneumoniae
NING Xi-bin; LIU Dai-xin; ZHANG Ji-lun
Pathogenesis and rapid detection of Vibrio parahaemolyticus