微生物与感染
  Home |  | About Journal | Editorial Board | Instruction | Publication Ethics | Subscriptions | Contacts Us | CHINESE
::: Office :::
Online Submission
Manuscript Tracking
Peer Review
Editor Work
Office Work
Editor-in-chief
 
::: Journal :::
Forthcoming Articles
Current Issue
Next Issue
Archive
Email Alert
 
Read Articles
Download Articles
  Quick Search  
 
  Advanced Search  
 
 
 
2008 Vol.3 No.1
Published 2008-03-25

述评
论著
Article
病例分析
讲座
综述
) [HTML 1KB] [PDF 82KB] ( 2043 )
 
论著
4

WANG Le-yao; ZHONG Peng; SHAN Liang; ZHONG Jiang

Experimental study on the co-application of Rta-expressing baculovirus vector and ganciclovir for the therapy of nasopharyngeal carcinoma

Objective To test a novel baculovirus expressing Epstein-Barr virus (EBV) lytic replication immediate early gene Rta, in combination with antiviral chemical ganciclovir, for the activation of latent EBV in nasopharyngeal carcinoma (NPC) cells, and the killing of tumor cells. Methods A baculovirus vector with EBV replication origin OriP, and the Rta expression cassette led by CMV promoter, was used to treat EBV-latently infected NPC cell line Hone1-EBV, as well as nude mice tumor model established from the cell line, in combination with ganciclovir. The effect on the growth of cell and tumor was studied. Results When treated with recombinant baculovirus in combinantion with ganciclovir, the growth of EBV-positive cells was significantly reduced to only 51% of the control. In the nude mice experiments, tumor growth was also greatly reduced. At 10th day after the first injection, the average volume and weight of the treated group was only about 20% and 30% of that of the control group, respectively. Histological analysis indicated there was significant cell necrosis in the baculovirus-ganciclovir-treated tumor tissue. Conclusion Co-application of RTA-expressing baculovirus vector and gancyclovir may be useful for the therapy of EBV associated NPC.

2008 Vol. 3 (1): 4-6 [Abstract] ( 2777 ) [HTML 1KB] [PDF 771KB] ( 2249 )
7

LI Xiao-xiao; MA Zhang-mei; WEN Yu-mei

Studies on hepatitis B virus genotypes and responses to antigen-antibody complex therapeutic vaccine

Objective To study whether there are differences in the responses of chronic hepatitis B patients infected with different hepatitis B virus (HBV) genotypes to HBsAg-HBIG therapeutic vaccine. Method Serum samples from sixty seven chronic hepatitis B patients who completed the full course of immunization with 60?g HBsAg-HBIG (YIC) were assayed for their virus genotypes by PCR-RFLP, in association with their viral and HBeAg sero-conversion responses to YIC treatment. Results Among the 67 patients, 22 were infected with HBV genotype B, and 43 were infected with genotype C, while in 2 patients the genotype of HBV could not be identified. In association with the responses to YIC treatment, no significant therapeutic differences were found between patients who were infected with HBV genotype B or with HBV genotype C. Conclusion Infections with HBV genotypes B or C do not affect the therapeutic responses in chronic hepatitis B patients to YIC.

2008 Vol. 3 (1): 7-10 [Abstract] ( 3125 ) [HTML 1KB] [PDF 196KB] ( 2305 )
11

LOU Qiang; ZHU Tao; Wang Jia-xue; WU Yang; Zhu Yu-li; QU Di

Methods of constructing Staphylococcus epidermidis gene mutants via different plasmids

Objective To construct Staphylococcus epidermidis gene knock-out mutants with different plasmids. Methods Recombinant plasmids were constructed for homologous recombination of the two component signal transduction system genes (arlS、saeRS and lytS) of S. epidermidis with two different shuttle plasmids, pMAD and pBT2, transformed to S. aureus RN4220 by electroporation and then transformed into S. epidermidis 1457. S. epidermidis mutants were selected using various antibiotics and biomarkers and identified by PCR and biochemistry experiments. Results With pMAD or pBT2 plasmid, we separately constructed S. epidermids genes knock-out homologous recombinant plasmids, pMAD-ΔsaeRS, pBT2-ΔarlS and pBT2-ΔlytS. S. epidermidis 1457 was transformed with the recombinant plasmids and the gene knocking out mutant was screened out, respectively. Only one cycle was needed for screening out SE1457-ΔsaeRS mutant and 5-10 cycles were required for screening out SE1457-ΔarlS and SE1457-ΔlytS mutant. The biofilm forming of SE1457-ΔarlS mutant decreased by 90.96% and no significant difference was observed between SE1457-ΔlytS、SE1457-ΔsaeRS mutants and SE1457 parent strain. Conclusion pMAD method can be used to construct S. epidermidis mutant strain simply and effectively.
2008 Vol. 3 (1): 11-16 [Abstract] ( 3524 ) [HTML 1KB] [PDF 315KB] ( 3055 )
17

WANG Fang; YAO Kun; FENG Dong-ju; ZHOU Feng; YIN Quan-zhang

Establishment of HHV-6-specific CD4+ T cell clones

Objective To establish the HHV-6-specific CD4+ T cell clones and further study the character of HHV-6-specific CD4+ T cells. Methods We acquired CD4+ T cell clones by the liquid microwell limiting dilution technique, and selected HHV-6-specific CD4+ T cell clones by means of 3H-thymidine uptake. The phenotype of HHV-6-specific T cell clones were analyzed with FACS. We tested the IL-10, IL-4 or IFN-γ secretion from HHV-6-specific CD4+ T cell clones by ELISA. Results Of the five HHV-6-specific CD4+ T cell clones, four showed proliferative responses to the stimulation with HHV-6 infected JJHAN lysates. Moreover, proliferation responses of HHV-6-specific CD4+T cell clones depended on the concentration of the HHV-6-infected JJHAN lysates. The phenotypic analysis with FACS demonstrated that HHV-6-specific T cell clones consisted of CD3+ CD4+ T cells. CD4+ T cell clones-W-2 and W-4 possessed the feature of high levels of IL-10 production, while CD4+ T cell clones-W-1 possessed the feature of high levels of IL-10 and IFN-γ production and W-3 possessed the feature of high levels of IFN-γ production. Conclusions The HHV-6-specific CD4+ T cell clones, were established in our study so as to further study their pneotype, the character of cytokine secrection, and to provide experimental basis for selecting HHV-6-specific CD4+ Treg cell clones.


2008 Vol. 3 (1): 17-20 [Abstract] ( 3385 ) [HTML 1KB] [PDF 198KB] ( 3463 )
21

Li Xiang-dong; Shen Yang; Qiu Ya-feng; Ma Zhi-yong

Meq protein of Marek’s disease virus inhibits the transactivity of p53

Objective To construct recombinant plasmids expressing wild type Meq (Meq-wt) and mutant Meq (Meq-mut) of Marek’s disease virus (MDV), and use the plasmids to explore the inhibitory activity of Meq gene on the transacitivty of p53. Methods Meq-wt was cloned by PCR, and Meq-mut, in which the p53 binding domain was deleted, was generated using PCR-based site-directed mutagenesis. The expressions of Meq-wt and -mut in primary chicken embryo fibroblasts (CEF) were detected by western blot. The p53-Luc report plasmid was co-tranfected with Meq-wt and -mut plasmids into CEF cells to evaluate the inhibitory activity of Meq gene on the transactivity of p53. The subcellular localizations of Meq proteins and p53 were also examined by immunofluorescent staining. Results DNA sequencing analysis showed that Meq-wt and –mut were obtained and inserted into the expression vector. The p53-Luc reporter gene assay showed that Meq inhibited the transactivity of p53. The Meq-wt protein was mostly localized in the nucleus of transfected cells and co-localized with p53 that is mainly localized in the nucleus, whereas the Meq-mut protein was distributed both in the cytoplasm and the nucleus. Conclusion Meq protein of MDV inhibits the transactivity of p53 and this inhibitory activity of Meq protein is exerted probably through directly binding to p53.
2008 Vol. 3 (1): 21-24 [Abstract] ( 3001 ) [HTML 1KB] [PDF 219KB] ( 2117 )
 
Article
25 XU Xiao; LU Ping; CHEN Gan; CHEN Gan-ying
Evaluation of the test of ADA and anti-TB in diagnosis of tuberculous pleural effusion

Objective To seek better diagnostic tests for tuberculous pleural effusion. Methods Adenosine deaminase (ADA) and anti-TB antibody in pleural effusion were tested by modified Martineck’s method and ELISA in 50 cases of tuberculous pleural effusion, 45 cases of malignant pleural effusion and 30 cases of right cardiac insufficiency pleural effusion, respectively. Result The level of ADA and anti-TB antibody in pleural effusion remarkably higher in tuberculous pleural effusion than in malignant pleural effusion and right cardiac insufficiency pleural effusion (p<0.01). When ADA >45U/L and positive anti-TB antibody were present,their sensitivity, specificity and accuracy in diagnosis of tuberculous pleural effusion are 100%, 98.6%, 99.2% and 90% , 93.3%, 92%, respectively. Conclusion The tests of ADA and anti-TB antibody in pleural effusion are very important in diagnosis tuberculous pleural effusion.

2008 Vol. 3 (1): 25-26 [Abstract] ( 3176 ) [HTML 1KB] [PDF 155KB] ( 3353 )
 
病例分析
27

CAI Xiao-di; CAO Yun; CHEN Chao; QIAN Tian; WANG Chuan-qing

Acinetobacter baumannii from ventilator-associated pneumonia in the NICU

Objective By analyzing Acinetobacter baumannii of Ventilator-associated pneumonia in NICU, we try to propose the strategy for prevention. Methods We collect the clinical characteristics and drug sensitivity results of the infants who suffered Acinetobacter baumannii of Ventilator-associated pneumonia from Jan 2006 to Feb 2007. Results Acinetobacter baumannii is the main pathogen of VAP , the epidemiology of which is complex. And the carbapenem-resistance strain was isolated from the infant in NICU, which will cause a worse prognosis. Conclusion To prevent Acinetobacter baumannii of VAP in NICU needs more attention of medical workers. Improved the surveillance on this kind of bacteria colony is important to prevent it and a further molecular epidemiology research on colonizing strains, especially the carbapenem-resistance strain will direct the prevention of it NICU.
2008 Vol. 3 (1): 27-29 [Abstract] ( 3192 ) [HTML 1KB] [PDF 216KB] ( 3047 )
 
讲座
30

LI Dan; LU Hong-zhou

New nomenclature for Pityrosporon ovale

2008 Vol. 3 (1): 30-31 [Abstract] ( 2085 ) [HTML 1KB] [PDF 122KB] ( 2551 )
 
综述
32

HUANG Chong; ZHANG Xin-xin

Research progress in hepatocacinoma secondary to hepatitis C virus infection

2008 Vol. 3 (1): 32-34 [Abstract] ( 1828 ) [HTML 1KB] [PDF 189KB] ( 1910 )
35

FEN Yan; GUO Xiao-kui

Helicobacter pylori and gastric cancer

2008 Vol. 3 (1): 35-38 [Abstract] ( 1882 ) [HTML 1KB] [PDF 190KB] ( 1920 )
39

CHEN Jing-xian; JIN Qi

Ebola virus

2008 Vol. 3 (1): 39-42 [Abstract] ( 1664 ) [HTML 1KB] [PDF 103KB] ( 1644 )
43

LI Wei-zhong; LI Qi-han

Herpes simplex virus type I protein ICP0: an important molecule regulating the interactions between the virus and host cells

2008 Vol. 3 (1): 43-46 [Abstract] ( 1961 ) [HTML 1KB] [PDF 117KB] ( 2135 )
47

YANG Yang; GUO Xiao-kui

Research progress of bacterial pore-forming cytotoxin

2008 Vol. 3 (1): 47-49 [Abstract] ( 1677 ) [HTML 1KB] [PDF 191KB] ( 2345 )
50

XIAN Mo; WU Zhong-dao

Involvement of innate immunity in anti-colonization caused by Streptococcus pneumoniae

2008 Vol. 3 (1): 50-52 [Abstract] ( 1694 ) [HTML 1KB] [PDF 190KB] ( 2155 )
53

NING Xi-bin; LIU Dai-xin; ZHANG Ji-lun

Pathogenesis and rapid detection of Vibrio parahaemolyticus

2008 Vol. 3 (1): 53-56 [Abstract] ( 1808 ) [HTML 1KB] [PDF 190KB] ( 4050 )
JOURNAL OF MICROBES AND INFECTIONS
Reader Login
Author Center
Online Submission
Author Instruction
Layout Art
Copyright Agreement
News



More >>  
Other Journal
Copyright © 2010  Editorial Board of Chinese Academy of Medical Sciences (CAMS) and the Peking Union Medical College (PUMC)
Add:Editorial office of Acta Academiae Medicinae Sinicae , No.9 Dongdansantiao, Beijing PRC(100730)
Fax:010-65133074 E-mail:actacams@263.net.cn
Supported by:Beijing Magtech