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2007 Vol.2 No.2
Published 2007-06-25

述评
论著
Review
病例分析
讲座
综述
) [HTML 1KB] [PDF 122KB] ( 1654 )
 
论著
68 MA Zhang-mei; TIAN Xiao-chen; LI Xiao-xiao; XU Dao-zhen; GUO Li-min; ZHAO Kai; WEN Yu-mei
Studies on hepatitis B virus S gene mutation in chronic hepatitis B patients treated with HBsAg-HBIG immunogenic complex therapeutic vaccine
Objective To study whether HBsAg-HBIG immunogenic complex therapeutic vaccine (YIC) could induce S gene immune escape mutants.Methods Five chronic hepatitis B patients who had been treated with 6 injections of either 30 or 60 micrograms of YIC were recruited for this study. These patients were those who responded to YIC treatment, shown as a drop in serum HBV DNA virus load >2 log10, with serum HBeAg converted to negative at 24 weeks. However, their virus load rebound at the end of 24 weeks follow-up. As controls, one was a patient who did not respond to YIC treatment and remained serum HBV DNA positive at similar level during and after treatment, while the other one was a patient immunized with the alum placebo. Serum samples before treatment and 6 months after termination of treatment were collected, and DNA extracted. PCR was used to amplify HBV S gene, the precore/core gene and the core promoter regions followed by sequencing.Results In all 7 patients treated with YIC or alum no S gene mutation was found in the “a” determinant, nor was precore stop codon mutation detected. However, three patients had mutations in the 1762/1764 core-promoter region, other two patients had mutations in the core promoter region other than the 1762/1764 double mutation.Conclusion The rebound of HBV replication in five YIC-treated patients was not due to the emergence of HBV S immune-escape mutants. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 68-72 [Abstract] ( 3237 ) [HTML 1KB] [PDF 965KB] ( 2202 )
73 LIN Shan-shan; WU Min; XU Yang; Xiong Wei; ZHANG Xiao-nan; YI Zhi-gang; YUAN Zhenghong
Inhibition of hepatitis B virus replication by MyD88 is mediated by nuclear factor-kappa B activation
Objective To investigate the mechanism of MyD88 inhibition of hepatitis B virus (HBV) replication. Methods Two truncated versions of MyD88, M (1-151) and M (152-296) were constructed. Plasmid IκBα-SR encoding NF-κB super-repressor or plasmid IKKα/IKKβ which can strongly activate NF-κB signaling were used. Huh7 cells were transiently transfected with HBV dimer, MyD88 or the above plasmids. Assays of HBsAg, HBeAg, core protein, HBV DNA and NF-κB activation were performed. Results The expression of the full length MyD88, and the two truncated forms, M (1-151) and M (152-296), showed different induction patterns of NF-κB activity; patterns that are consistent with their inhibitory effect on viral protein synthesis and core particle-associated HBV DNA replication. Similarly, the expression of MyD88 resulted in a significant reduction of core protein and the co-expression of IKKα/IKKβ dramatically inhibited the core protein level. Furthermore, the co-expression of IκBα-SR dramatically restored the core protein level. Similar results were also observed for HBeAg, HBsAg and HBV core particle DNA. Conclusion Results from these studies support a role of NF-κB signaling activation in HBV viral replication and suggest a novel mechanism for the inhibition of HBV replication by MyD88. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 73-77 [Abstract] ( 3260 ) [HTML 1KB] [PDF 530KB] ( 2328 )
78 SHAO Sheng-wen; YU Jian-guo; WU Wen-bin; ZHAO Ping; DING Hui; QI Zhong-tian
Intercellular distribution of hepatitis C virus F protein is associated with its mutation in phosphorylation sites
Objective To determine the relationship between phosphorylation site mutation and intercellular distribution of hepatitis C virus (HCV) F protein in HepG2 cells.Methods According to the phosphorylation sites of HCV F protein as predicted by NetPhos2.0 Server software, overlapping primers were designed and used to synthesize the HCV mutated f (mf) gene, in which the codons 13, 14, 114, 121 and 124 were mutated from TCT to GAT. Consequently, the amino acids shifted from serine(S) to aspartate (D). The recombinant pEGFP-mf and pEGFP-f were constructed by cloning the mf gene and f gene into pEGFP-N1, respectively. The resulting recombinant plasmids were transfected into HepG2 cells by liposome transfection method, and the distribution of mutant F protein or wild-type F protein in HepG2 cells was examined by laser confocal microscope.Results In HepG2 cells, the mutant F protein was located mainly in the nucleus (90%) and only 10% was in the cytoplasm. The mean fluorescence intensity of mutated F protein was significantly different between the nucleus and cytoplasm 〔63.70±3.20 and 7.06±0.34, respectively; (P<0.001,t=99.2)〕. On the contrary, the wild-type F protein was located mainly in the cytoplasm(94.9%) and only a small fraction was in the nucleus (5.1%). The mean fluorescence intensities for the wild-type F protein in the cytoplasm and nucleus were 83.34±4.07 and 4.48±0.22, respectively (P<0.001, t=106.5). Conclusions Phosphorylated and non-phosphorylated forms of the HCV F protein have different cellular distributions. The phosphorylated F protein lies mainly in cytoplasm and is involved in the regulation of HCV replication whereas the non-phosphorylated F protein exists mainly in the nucleus and may effect on gene transcription. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 78-82 [Abstract] ( 2622 ) [HTML 1KB] [PDF 390KB] ( 2414 )
83 WU Xiao-cheng; MIAO Ji; ZHENG Zi-zheng; HE Shui-zhen; SUN Yuan-yuan; TANG Ming; ZHANG Jun; XIA Ning-shao
Grp78/Bip facilitates the attachment/entry of the hepatitis E virus capsid protein to host cells
Objective To further investigate the interaction between recombinant hepatitis E virus (HEV) capsid protein p239 and Grp78/Bip and the role of Grp78/Bip in HEV penetration.Methods We utilized pull-down, immunoprecipitation and antibody blocking assays to examine the interaction between p239 and Grp78/Bip. Confocal microscopy was used to investigate the co-localization of these two proteins. Purified Grp78/Bip was used to block the attachment of p239 to host cells.Results p239 directly bound to Grp78/Bip and this binding was sensitive to ATP. Furthermore, antibody blocking results demonstrate that this interaction was indeed conformation-dependent. A partial co-localization of p239 and Grp/Bip was observed on the plasma membrane of HepG2 by confocal microscopy. Pre-incubation of Grp78/Bip with p239 significantly blocked the attachment of p239 to HepG2 cells.Conclusion Grp78/Bip participates in the attachment and/or entry of the HEV capsid protein to host cells. These results further contribute to the understanding of the entry mechanism of the hepatitis E virus after infection. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 83-87 [Abstract] ( 2746 ) [HTML 1KB] [PDF 441KB] ( 2363 )
88 XU Jian; YAO Kun; DOU Jie; XU Wen-rong; YIN Quan-zhang; CHEN Yun; ZHOU Feng
Cloning and expression of human herpesvirus 7 pp85 and corresponding antibody production
if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 88-91 [Abstract] ( 2638 ) [HTML 1KB] [PDF 475KB] ( 1912 )
92 GONG Qi-ming; QIU De-qi; Lu Zhi-meng; ZHANG Xin-xin
The analysis of clinical characteristics in 77 HIV infected individuals
2007 Vol. 2 (2): 92-94 [Abstract] ( 2700 ) [HTML 1KB] [PDF 323KB] ( 1822 )
99 ZHANG Qun; FU Zhi-ren; CAI Jian-fei; TANG Xiao-feng; PENG Jie-jie
Analysis of the risk factors associated with fungal infection in liver transplant recipients
Objective The goal of the current study was to determine the characteristics of fungal infection in liver transplant recipients, analyze the associated risk factors, and to further examine prophylactic measures taken to prevent such infections. Methods One hundred and ninety five liver transplant recipients were retrospectively studied in the Liver Transplant Center between January 2003 to January 2005. Results Fungal infection occurred in 25 of 195 recipients with the morbidity rate of 12.7%. Forty strains of fungi were isolated including 25 cases of Candida albicans (62.5%), 9 cases of C. glabrata (22.5%), 5 cases of C. tropicalis (12.5%) and 1 case of C. guilliermondii (2.5%). The majority of infections occurred in the respiratory tract (49%). Compared to the control group, the most common risk factors associated with fungal infection in liver transplant recipients were (1) long-term administration of broad-spectrum antibiotics both pre-and post-operation, (2) the total number of days the patient stayed in the hospital, (3) the amount of time a vein catheter had to remain in place, (4) the counts of white blood cells and neutrophil cells 24 hours post-operation, (5) the levels of the total and direct bilirubin and (6) the total volume of blood received during the operation. Three out of 195 recipients (12%) died as a result of fungal infection. Conclusion Multiple risk factors can lead to a fungal infection after liver transplantation. Results from this study indicate that certain preventative measure must be taken in order to eliminate or reduce the severity and/or occurence of fungal infections following liver transplantation including narrowing the course of broad spectrum antibiotics, adopting an strict indication of vein puncture, shortening the number of days a vein catheter stays in place, and post-operation monitoring white blood cell counts and hepatic function. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}
2007 Vol. 2 (2): 99-101 [Abstract] ( 2830 ) [HTML 1KB] [PDF 153KB] ( 1806 )
 
Review
95 LI Yue-fang; ZENG Mei; ZHU Qi-rong; WANG Xiao-hong
Clinico-epidemiological characteristics of mumps in 890 children from Shanghai region in 2005[J]. 微生物与感染, 2007,2(2): 95-98 >')" href="#"> Clinico-epidemiological characteristics of mumps in 890 children from Shanghai region in 2005

Objective To understand the current epidemiological status, clinical futures and economic burden of mumps infection in children in the Shanghai area.Methods A retrospective cross-sectional clinical study was conducted to analyze clinical data from children with mumps including 890 outpatient and 270 inpatient cases occurring in 2005.Results Mumps infections were prevalent throughout the whole year in Shanghai with infections peaking from April through July. Among the 270 inpatient cases, the majority (67.8%) of cases occurred in children of families who were originally living outside Shanghai. Only eight (3.0%) inpatients had received one dose of MMR vaccine. The majority of children with mumps in this inpatient population were older than 3 years. Among the 890 outpatient cases, 234 (26.3%) experienced complications and were admitted to the isolation wards. Complications included meningitis and/or encephalitis (87.6%), orchitis(6.0%),pancreatitis(4.7%),and deafness (0.4%). All patients survived. The direct medical costs for each outpatient clinic visit were between 114.9 and 184.7 Yuan averaging 142.9±20.5 Yuan. The average number of visits was 1.4 per patient. In contrast, the direct medical costs for inpatient admission ranged from 624.6 Yuan to 6190.2 Yuan with costs averaging 1 897.8±744.2 Yuan. Conclusion Mumps is a common communicable disease for children in Shanghai that imposes a threat on children's health. Children attending school are a high-risk, susceptible population. Results from this study show that the mumps vaccine should be routinely administered to children in order to protect them against mumps infection and decrease the complications and financial burdens of this disease.

2007 Vol. 2 (2): 95-98 [Abstract] ( 2603 ) [HTML 1KB] [PDF 328KB] ( 2688 )
 
病例分析
102 LU Hong-zhou;QI Xun
2007 Vol. 2 (2): 102-103 [Abstract] ( 1890 ) [HTML 1KB] [PDF 121KB] ( 2047 )
104 LU Hong-zhou;QI Xun
2007 Vol. 2 (2): 104-106 [Abstract] ( 1869 ) [HTML 1KB] [PDF 94KB] ( 1860 )
 
讲座
107 CHEN Hui;YUAN Zheng-hong
2007 Vol. 2 (2): 107-110 [Abstract] ( 1836 ) [HTML 1KB] [PDF 184KB] ( 2690 )
 
综述
111 JIA Ni-na;XIE Qing
2007 Vol. 2 (2): 111-114 [Abstract] ( 1420 ) [HTML 1KB] [PDF 190KB] ( 2893 )
115 LIN Zhi-mei;XIE Qing
2007 Vol. 2 (2): 115-117 [Abstract] ( 1538 ) [HTML 1KB] [PDF 154KB] ( 1604 )
118 CHEN Ding-qiang;ZHENG Bo-jian
2007 Vol. 2 (2): 118-119 [Abstract] ( 1446 ) [HTML 1KB] [PDF 123KB] ( 1483 )
120 HU Zhi-peng;ZHONG Jiang
2007 Vol. 2 (2): 120-124 [Abstract] ( 1476 ) [HTML 1KB] [PDF 251KB] ( 4197 )
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