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::: Office ::: Online Submission Manuscript Tracking Peer Review Editor Work Office Work Editor-in-chief   ::: Journal ::: Forthcoming Articles Current Issue Next Issue Archive Email Alert   Read Articles Download Articles   Quick Search       Advanced Search         2020 Vol.15 No.2 Published 2020-04-25 Invited paper Original Article Review ) [HTML 1KB] [PDF 637KB] ( 818 )   Original Article 76 YAO Mengyi, Qian Jianing, DI Yuchang, CHEN Run, BAI Jiacheng, ZHANG Xuelian Phenotypes of HA37Ra isolates resistant to novel antitubercular compounds ATB-152E and ATB-152J are structurally similar anti-tuberculosis (anti-TB) chemicals developed by our group in previous research. To study their mechanisms, Mycobacterium tuberculosis (M. tuberculosis) H37Ra strains spontaneously resistant to them were collected and subjected to colony morphology analysis and biofilm formation assay. In total, seventeen ATB-152E resistant strains and fifteen ATB-152J resistant strains were studied. The frequencies of resistance to both compounds were 10－7. Compared with the wild type strains of H37Ra, ATB-152E resistant strains had more folds, while ATB-152J resistant strains presented a flatter pattern with reduced folds. Meanwhile, the time of biofilm formation of resistant strains also differed from the wild type strains, indicating a possible mechanism of inhibition via lipid metabolism. The acquisition of the strains resistant to ATB-152E and ATB-152J could lay a foundation to further study of them. 2020 Vol. 15 (2): 76-81 [Abstract] ( 50 ) [HTML 1KB] [PDF 10471KB] ( 372 ) 82 HUANG Guixian1,2, LIANG Shanshan2, PENG Ying1,2, SHEN Hongbo2, WANG Feifei3, XU Junfa1 miR-125b-5p regulates mycobacteria growth in host cells and in mice MicroRNAs (miRNAs) play an important role in the regulation of the immune system. To demonstrate the role of miR-125b-5p in anti-TB immunity, we have infected host target lung alveolar A549, human macrophage THP-1, mice macrophage RAW264.7 and C57BL/6 mice, respectively. The q-PCR results demonstrated that miR-125b-5p is highly expressed in the tested conditions. The expression of miR-125b-5p in BCG infected mice lung was about 15 times of that in the control mice. The results verified that miR-125b-5p regulated mycobacteria growth in A549, THP-1 and RAW264.7 cells, respectively. Moreover, administration of miR-125b-5p inhibitors in BCG infected mice significantly reduced bacteria burden in lung. Thus, we concluded that miR-125b-5p may play a critical role on mycobacteria proliferation in vivo. The results provide useful information for our understanding of host immune defense and potential therapeutic interventions against this disease. 2020 Vol. 15 (2): 82-87 [Abstract] ( 58 ) [HTML 1KB] [PDF 1998KB] ( 387 ) 88 ZHOU Yiqing1, RUAN Yongchun1, ZHOU Jie1, ZHANG Haiwang1, SU Meixia1, ZENG Min1, WANG Dan1, MAO Guofeng2, LI Minghui1 Hfq protein characteristics and effects on drug resistance of Klebsiella pneumoniae Host factor for RNA phage QB replicase (Hfq) is a global regulator that regulates sRNA-mRNA binding and stability and is involved in bacterial growth, chemotaxis, virulence, drug resistance, and response to external stress. The hfq gene of 59 clinical isolates of Klebsiella pneumoniae (KP) collected from Aug 2017 to Aug 2018 in our hospital were extracted and compared with the ones downloaded from NCBI database. Transeq, multiple sequence alignments of amino acid sequences were performed with MAFFT software, and the secondary structure of the protein were analyzed using the online website ESPript3.0. The physicochemical properties of the amino acid sequences were calculated using the online website ProtParam. Hfq domains were predicted from Conserved domains in the NCBI database. The tertiary structure of Hfq protein was predicted using Phyre2. The CRISPR/Cas9 homologous recombination technology was used to knock out the hfq gene in KP strain. The wild strain and the hfq knockout strain were diluted by 1∶100, and 0.1 μg/mL, 0.2 μg/mL, and 0.3 μg/mL Imipenem/cilastatin sodium were added to co-culture respectively. The growth curve was drawn by taking the 1 h, 2 h, 3 h, 4 h bacteria liquid count respectively. Despite 5 hfq gene mutations detected in 3 strains, the amino acid sequences of all strains were identical. The tertiary structure prediction indicated that 68 (66.67%) amino acids were consistent with the template sequence, and the more conserved functional domain was located at the 54-59 sites (VYKHAI). The hfq gene of one clinical strain was successfully knocked out by CRISPR/Cas9 homologous recombination. The resistance of the hfq knockout strain to antibiotic significantly decrease than that of the wild type(P<0.05). The results of this study suggest that KP’s Hfq amino acid sequence is conserved, and the gene may play a role in carbapenems resistance. 2020 Vol. 15 (2): 88-97 [Abstract] ( 76 ) [HTML 1KB] [PDF 4389KB] ( 727 ) 98 YAO Hailan1, SONG Juan2, WANG Xinling2, WANG Ruifang2, SONG Qinqin2, SHI Bintian2, HAN Jun2 The change of endogenous siRNAs in coxsackie virus type B3-infected HeLa cells To explore whether coxsackie virus type B3 (CVB3) infection can induce the expression of endogenous siRNA, siRNAs were analyzed in CVB3-infected HeLa cells at 3h and 6h post-infection (pi) by the high-throughput sequencing. The results showed that CVB3 induced 14 endogenous siRNA and 5 endogenous siRNA in HeLa cells at 3 h and 6 h pi, respectively. Two sustainably expressed endogenous siRNAs were further confirmed at 3 h and 6 h pi using quantitative PCR. Results of sequence homology alignment showed that two siRNAs could recognize the precursor RNA of 45S ribosome and 28S ribosome. The results suggest that CVB3 infection may interfere ribosome maturation. 2020 Vol. 15 (2): 98-103 [Abstract] ( 60 ) [HTML 1KB] [PDF 728KB] ( 700 )   Review 104 YANG Hao1，2, WU Yonglin1，2, RONG Xingyu1，2, YAN Yuchen1，2, WANG Huijing3, NIU Chen1，2, ZHAO Chao1，2 Re-understanding the potential mechanism of angiotensin converting enzyme 2 in severe acute respiratory syndrome coronavirus 2 pathogenicity from the perspective of gut microbiota Since the out breaking of coronavirus induced disease 2019 (COVID-19), the etiology and clinical manifestations of the disease have been reported and analyzed. There are many similarities between the etiology and clinical manifestations of the SARS in 2003. By comparing the similarities and differences between them, this study attempts to put forward and explore the potential mechanism of gut microbiota in patients, which may participate in its pathogenesis from the perspective of the shared receptor angiotensin converting enzyme 2 (ACE2). It may provide a possible way for in-depth study of the pathogenesis of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) and the development of prediction indicators for severe pneumonia. 2020 Vol. 15 (2): 104-107 [Abstract] ( 142 ) [HTML 1KB] [PDF 404KB] ( 357 ) 108 PAN Qingchun, TANG Zhenghao, YU Yongsheng, XI Min, ZANG Guoqing Influenza prevention and control under the new urbanization situation 　 Influenza is a serious threat to public health. Comprehensive strategies, including strengthening virus surveillance, recommendation and promotion of vaccination, conducting outbreak investigation and control, etc. are effectively used to prevent and control influenza. New urbanization, with rapid transportation and large-scale urban population poses enormous challenges to the current system of influenza prevention and control. New measures and strategies are required. 2020 Vol. 15 (2): 108-114 [Abstract] ( 44 ) [HTML 1KB] [PDF 859KB] ( 471 ) 115 WANG Shilin, MENG Tianjiao, SHANG Qinglong Progress on oncolytic mechanism of enteroviruses As infection by enteroviruses being asymptomatic or causing mild lesions in human beings, enterovirus-based oncolytic viruses have received extensive attentions. A large number of experimental results demonstrated that it is safe and effective way to use enterovirus to treat tumor. The oncolytic mechanism of different enterovirus remains open. In this paper, the molecular mechanism of oncolytic enterovirus, modification of enterovirus and clinical trials are reviewed. 2020 Vol. 15 (2): 115-121 [Abstract] ( 53 ) [HTML 1KB] [PDF 642KB] ( 589 ) 122 CHEN Zhu, CHEN Junhua Advances in research on the relationship between tuberculosis and gut microbiome In recent years, with the development of next-generation sequencing technology, gut microbiome has become a hot spot in the field of biological research. The relationship between gut microbiome and various diseases has got widespread attention. It has been found that Mycobacterium tuberculosis infection causes changes of the gut microbiome. At the same time, dysbiosis of the gut microbiome also increases the susceptibility to tuberculosis. Immune response is an important factor affected by metabolic products produced by gut microbiome. This article reviews studies on the relationship between tuberculosis and gut microbiome, the possible mechanism of the interaction between them, and the impact of antituberculosis treatment on gut microbiome. 2020 Vol. 15 (2): 122-128 [Abstract] ( 45 ) [HTML 1KB] [PDF 676KB] ( 609 ) 129 LIU Ruijie1, WANG Xinxia1, WANG Lu1, ZHAO Ziyao1, YU Chihao1, YU Hong2, YANG Xuejing3, SUN Guiqin1 Advances in suppurative otitis media caused by pathogenic infections Suppurative otitis media is a common disease in the department of otolaryngology, which would result in hearing loss, tympanic membrane congestion, tympanic membrane perforation, tinnitus, ear pain and purulent clinical symptoms. Suppurative otitis media is mainly associated with microorganisms that could cause the middle ear infection and the middle ear suppurative lesions. In this paper, the pathogenesis of suppurative otitis media, pathogenic bacteria, drug resistance and treatment methods were summarized to provide evidence for clinical diagnosis and rational drug use of suppurative otitis media. 2020 Vol. 15 (2): 129-134 [Abstract] ( 53 ) [HTML 1KB] [PDF 745KB] ( 459 ) Reader Login Author Center Online Submission Author Instruction Layout Art Copyright Agreement News More >>   Other Journal

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