The purpose of this study is to analyse the hepatitis B virus（HBV） serological characteristics in occult hepatitis B virus infection (OBI) among blood donors. We selected 395 nucleic acid test positive blood samples and 523 nucleic acid test negative blood samples from 2013 to 2018 in Shanghai Blood Center, which were with normal ALT and with HBsAg，anti-HCV and HIV antigen-antibody negative. Nucleic acid test positive samples were tested by HBV DNA identification reagent with PCR or transcription-mediated amplification ( TMA) essays. HBV serological markers were tested by chemiluminescent method in all of the samples. We found that among 395 nucleic acid test positive samples, 197 were positive for HBV DNA identification, which were considered to be OBI. Most of the OBI samples were positive for anti-HBc (85.79%). The main serological patterns in OBI were anti-HBs positive and anti-HBc positive or anti-HBc positive alone. The main serological patterns in the qualified blood donors were seronegative and anti-HBs positive,. The level of anti-HBs in the OBI group was significantly lower than that in the qualified blood donors group (Z =-0.198, P < 0.05). The level of anti-HBs in OBI group was mainly lower than 100 mIU / mL. However, the proportion of high anti-HBs level (> 1000 mIU/mL) in qualified blood donor group was higher than that in the OBI group (P < 0.05). It indicated that the HBV serological markers in most of the occult HBV infected people in Shanghai area are different from the qualified blood donors.