Facing the outbreak of COVID-19, whether biosafety level 3 (BSL-3 ) laboratory can be immediately started in time and operate safely providing a reliable platform for COVID-19 virus research and detection, is ordeal for a laboratory's ability meeting with an emergency. After receiving the mission, BSL-3 laboratory of Fudan University immediately started the application process for new coronavirus experiment activities in the lab, following the regulations of China. After obtained the approval from the National Accreditation Service for Conformity Assessment and the National Health Commission, New coronavirus strains were isolated from a sample of Shanghai COVID-19 patient within one week, which reflecting the importance of laboratory’s connotation construction in peacetime. The BSL-3 laboratory’s connotation construction includes the biosafety management systems such as risk assessment and management of laboratory, personnel training, and management of facilities and equipment, ect. In this article, we shall introduce the experience and comprehension gained from management of the Fudan University BSL-3 laboratory.