Brucellosis is an infectious-allergic zoonotic disease caused by Brucella. It is easily neglected. This disease seriously threatens human health, affects the development of animal husbandry and tourism, hampers normal international trade, and will cause hidden food safety hazards. Brucella can be transmitted to humans through multiple ways, including gastro-enteral tract, respiratory tract, and damaged skin and mucous membranes. There is no age or gender difference in the susceptibility of humans to Brucella. Humans can be infected with Brucella through direct or indirect contact. Laboratory-acquired brucellosis also occurs in laboratories dealing with Brucella, but it can be prevented with effective biosafety measures. Brucellosis is a preventable and controllable disease. It needs early diagnosis and proper treatment for preventing from chronic brucellosis. This review introduces the biosafety, prevention and control of brucellosis.