微生物与感染
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微生物与感染
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51例新生儿脐带血单纯疱疹病毒的检测
任鹏1,姚娟1,金艳2,李法锦1,汪晓婷1,葛俊1,江龙凤1,赵蕾1,吉阳1,王明丽1
1.安徽医科大学微生物学教研室,合肥 230032;2.合肥市妇幼保健院,合肥 230001
Detection of herpes simplex virus infection in umbilical cord blood in 51 neonates
REN Peng1, YAO Juan1, JIN Yan2, LI Fa-Jin1, WANG Xiao-Ting1, GE Jun1, JIANG Long-Feng1, ZHAO Lei1, JI Yang1, WANG Ming-Li1
1. Department of Microbiology, Anhui Medical University, Hefei 230032,China; 2.Hefei Maternity and Child Health Hospital, Hefei 230001, China
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摘要: 为了解健康产妇顺产新生儿单纯疱疹病毒(HSV)感染情况,自2010年11月~2011年6月于某地妇幼保健院采集顺产新生儿抗凝脐带血51份。提取新生儿脐带血单核细胞DNA后,利用HSV-1 gD基因和HSV-2 gG基因特异性引物,进行套式聚合酶链反应(PCR)检测,并将阳性扩增产物测序后进行BLAST比对确认,分析HSV感染阳性率。结果显示,51份脐带血HSV-1阳性12份,感染率23.53%; HSV-2阳性13份,感染率25.49%;HSV-1和HSV-2共阳性4份,重复感染率7.84%。结果表明,加强新生儿HSV感染的早期诊断和治疗,对预防新生儿HSV感染所致疾病,防止后遗症具有重要意义。

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作者相关文章
任鹏
姚娟
金艳
李法锦
汪晓婷
葛俊
江龙凤
赵蕾
吉阳
王明丽
关键词 单纯疱疹病毒感染脐带血新生儿    
Abstract:The present paper aims to investigate the herpes simplex virus (HSV) infection in neonates. From November 2010 to June 2011, fresh neonatal cord blood was collected from 51 neonates in a Maternity and Children Hospital and transported immediately to our laboratory. After DNA extraction from the samples, nested-polymerase chain reactions (PCR) were performed by using HSV-1 gD gene- and HSV-2 gG gene-specific primers to detect congenital HSV-1 and HSV-2 infections respectively. The PCR products were sequenced toconfirm the HSV infections.. The results indicated that the infection rate of HSV-1 and HSV-2 were 23.53% and 25.49% respectively. The HSV-1/HSV-2 co-infection rate was 7.84%. This study suggests that early diagnosis and treatment of neonatal HSV infection is critical for the prevention of diseases caused by HSV infection.

Key wordsHerpes simplex virus infection    Umbilical cord blood    Neonate
通讯作者: 王明丽   
引用本文:   
任鹏1,姚娟1,金艳2,李法锦1,汪晓婷1,葛俊1,江龙凤1,赵蕾1,吉阳1,王明丽1. 51例新生儿脐带血单纯疱疹病毒的检测[J]. 微生物与感染 , 2012, 7(2): 95-98.
REN Peng1, YAO Juan1, JIN Yan2, LI Fa-Jin1, WANG Xiao-Ting1, GE Jun1, JIANG Long-Feng1, ZHAO Lei1, JI Yang1, WANG Ming-Li1. Detection of herpes simplex virus infection in umbilical cord blood in 51 neonates. Journal of Microbes and Infections, 2012, 7(2): 95-98.
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http://jmi.fudan.edu.cn/CN/      或     http://jmi.fudan.edu.cn/CN/Y2012/V7/I2/95

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