微生物与感染
2024年11月28日 星期四   首页 |  主编心语 |  期刊介绍 |  编委阵容 |  投稿指南 |  出版道德 |  期刊订阅 |  联系我们 | English
微生物与感染  2016, Vol. 11 Issue (4): 228-233    
  论著 本期目录 | 过刊浏览 | 高级检索 |
5-氨基酮戊酸光动力疗法对不同疾病来源的耐甲氧西林金黄色葡萄球菌的体外杀伤作用
赵可庆1,杨晨1,2,吴旸3,郭丽敏1,刘华勇3,吉建4,陈小英5,郑春泉1
1. 复旦大学附属眼耳鼻喉科医院耳鼻咽喉科,上海市耳鼻咽喉科临床重点学科,上海200031;2. 上海交通大学医学院附属瑞金医院耳鼻咽喉科,上海200025;3. 复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生部重点实验室,上海200032;4. 复旦大学附属眼耳鼻喉科医院检验科,上海200031;5. 上海交通大学医学院附属瑞金医院皮肤科,上海200025
In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases
ZHAO Keqing1, YANG Chen1,2, WU Yang3, GUO Limin1, LIU Huayong3, JI Jian4, CHEN Xiaoying5, ZHENG Chunquan1
1. Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University; Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai 200031, China; 2. Department of Otolaryngology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine,, Shanghai 200035, China; 3. Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; 4 . Department of Clinical Laboratory, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China; 5. Department of Dermatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200035, China
全文: PDF (1537 KB)   HTML (0)
输出: BibTeX | EndNote (RIS)      
摘要: 光动力疗法(photodynamic therapy,PDT)利用光敏剂与光源反应后产生的活性氧,破坏细菌组分,进而致细菌死亡。其多靶位杀伤特性在治疗耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)感染方面有应用前景,但相关研究尚处于起步阶段。本研究MRSA菌株取自烧伤、急性咽炎、鼻窦炎和肺炎4类临床常见MRSA感染性疾病患者,使用5-氨基酮戊酸(5-aminolevulinic acid,ALA)光敏剂、发光二极管光源,于体外检测ALA介导的PDT(ALA-PDT)对MRSA菌株的杀伤作用。结果显示,经5 mmol/L ALA孵育1 h后,给予360 J/cm2强光〔(633±10)nm〕照射1 h,ALA-PDT对MRSA菌株具有1.80 log10 cfu的有效杀伤作用。结果提示,在相同实验条件和参数下,ALA-PDT对上述4种疾病来源的MRSA菌株体外杀伤作用无统计学差异。

服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
赵可庆1
杨晨1.2
吴旸3
郭丽敏1
刘华勇3
吉建4
陈小英5
郑春泉1
关键词 光敏疗法5-氨基酮戊酸耐甲氧西林金黄色葡萄球菌    
Abstract:In photodynamic therapy (PDT), the reactive oxygen species generated by the activation of photosensitizers by light of an appropriate wavelength, are able to inactivate microbes by damaging various microbial components. Due to its multi-targeting antimicrobial capability, PDT has shown a potential as an effective alternative therapeutic option against methicillin-resistant Staphylococcus aureus (MRSA). In this study, MRSA strains isolated from burn, acute pharyngitis, nasosinusitis and pneumonia patients were subjected to a 5-aminolevulinic acid (ALA) mediated light emitting diode (LED) array in vitro. The results showed that a significant reduction (1.8 log10 cfu) was achieved with an ALA treatment (1 h, 5 mmol/L) followed by 1 h of illumination [(633±10) nm wavelength light source, and a 360 J/cm2 light dose]. MRSA strains collected from different tissues showed a similar sensitivity to the treatment.

Key wordsPhotodynamic Therapy    5-Aminolevulinic acid    Methicillin-resistant Staphylococcus aureus
基金资助:国家自然科学基金(81300810),上海市青年医师培养资助计划(20141057)

通讯作者: 郑春泉,赵可庆   
引用本文:   
赵可庆1,杨晨1.2,吴旸3,郭丽敏1,刘华勇3,吉建4,陈小英5,郑春泉1. 5-氨基酮戊酸光动力疗法对不同疾病来源的耐甲氧西林金黄色葡萄球菌的体外杀伤作用[J]. 微生物与感染, 2016, 11(4): 228-233.
ZHAO Keqing1, YANG Chen1.2, WU Yang3, GUO Limin1, LIU Huayong3, JI Jian4, CHEN Xiaoying5, ZHENG Chunquan1. In vitro study of antimicrobial effect of photodynamic therapy using 5-aminolevulinic acid-mediated photodynamic treatment against methicillin-resistant Staphylococcus aureus strains from different infectious diseases. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(4): 228-233.
链接本文:  
http://jmi.fudan.edu.cn/CN/     或     http://jmi.fudan.edu.cn/CN/Y2016/V11/I4/228

版权所有 © 2008 《微生物与感染》编辑部
地址:上海市医学院路138号 邮编:200032
Tel:021-54237633 Fax:021-64434224    E-mail:jmi@fudan.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn