Abstract：The present paper aims to investigate the role of interleukin 23 (IL-23) in facilitating Th1, Th2 and Th17 differentiation during respiratory syncytial virus (RSV) infection of epithelial cells (BEAS-2B). Lymphocytes were treated by supernatants from BEAS-2B cells with RSV or mock infection. Then they were blocked by specific anti-IL-23R antibody, anti-IL-23p19 antibody and p38 mitogen-activated protein kinase (MAPK) inhibitor (SB203580). The concentrations of cytokines such as interferon γ (IFN-γ), IL-4 and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of transcription factors (t-bet, gata3, rorγt), signal transducers (stat4, stat6, stat3) were determined by real-time polymerase chain reaction (PCR). The results showed that the concentrations of cytokines (IFN-γ， IL-4 and IL-17) were significantly increased after RSV infection, accompanied with the enhanced expressions of transcription factors. However, these cytokines and transcription factors were significantly decreased when IL-23 pathway was blocked by antibodies. The blockage of p38 MAPK signal pathway showed the same results. The results suggest that IL-23 could facilitate Th1, Th2 and Th17 differentiation in RSV-infected BEAS-2B cells, which might be associated with p38 MAPK signal pathway.
冯净净，陈家君，王盛美，揭志军. 白细胞介素23在呼吸道合胞病毒感染致Th1、Th2及Th17细胞分化中的作用及机制[J]. 微生物与感染, 2017, 12(5): 279-286.
FENG Jingjing, CHEN Jiajun, WANG Shengmei, JIE Zhijun. Role of interleukin 23 in facilitating Th1, Th2 and Th17 differentiation after respiratory syncytial virus infection. JOURNAL OF MICROBES AND INFECTIONS, 2017, 12(5): 279-286.