Coxsackievirus B3 2A protease blocks nuclear translocation of nuclear factor κB (NF-κB) and inhibits dimerization of NF-κB
SHENG Lin-Jun, SONG Qin-Qin, LU Ming-Zhi, SONG Juan, SUN Peng, WANG Bao-Dong, CHI Miao-Miao, HAN Jun
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China Center for Diseases Control and Prevention, Beijing 102206, China
Abstract：To study the impact of coxsackievirus B3 2A protease on nuclear factor κB (NF-κB ), BHK cells were transfected with a plasmid pcDNA3.1-2A expressing CVB3 2A protease along with pGL3-NF-κB -promotor-luc. The activity of luciferase was detected to identify the relative effect of NF-κB upon tumor necrosis factor α (TNF-α) stimulation. The dimerization of NF-κB /p65 were also detected with Western blotting and translation of NF-κB /p65 was observed by immunofluorescence. The results showed that CVB3 2A protease not only inhibited the expression of luciferase activities from NF-κB /p65 promoter but also interfered the nuclear translocation of NF-κB /p65. CVB3 2A protease still inhibited the dimerization of NF-κB /p65. It is concluded that CVB3 protease 2A inhibits the activation of NF-κB pathway.
盛琳君，宋芹芹，卢明枝，宋娟，孙鹏，王宝栋，迟苗苗，韩俊. 柯萨奇病毒B3蛋白酶2A干扰核因子κB p65的二聚体形成和核转移[J]. 微生物与感染, 2014, 9(3): 147-151.
SHENG Lin-Jun, SONG Qin-Qin, LU Ming-Zhi, SONG Juan, SUN Peng, WANG Bao-Dong, CHI Miao-Miao, HAN Jun. Coxsackievirus B3 2A protease blocks nuclear translocation of nuclear factor κB (NF-κB) and inhibits dimerization of NF-κB. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(3): 147-151.
