Abstract：Laboratories—General Requirements for Biosafety（GB 19489-2008） required “Exhaust high efficiency particulate air (HEPA) filter of Biosafety Level 3 Laboratory (BSL-3) should be sterilized in situ”. Exhaust HEPA filter is an important defensive line to ensure the biosafety of BSL-3 laboratory facilities and also the main line of defense to ensure the non-leakage of infectious aerosols generated in the experimental procedure. In situ disinfection of the HEPA filter is needed in order to protect the safety of personnel. This study designed and produced a cycle disinfection prototype (with the function of formalin volatilization, gas humidification and air circulation supply). The prototype with the disinfection interface of HEPA filter’s exhaust box were connected. And then the steam cycle of formalin was driven through the HEPA filter by air circulation supply system. Thus the in situ disinfection was realized. The results showed that formalin vapor concentration at room temperature was 16 mg/L, and the relative humidity was 70%. After 12-hour continuous fumigation cycle running through, Staphylococcus aureus, Mycobacterium smegmatis, Bacillus subtilis and Bacillus stearothermophilus were completely disinfected. As a consequence, the in situ disinfection of HEPA exhaust-air filter in both equipment and methodology is achieved.
韩文东1; 孙志平1; 丁悦娜1; 曹明波2; 瞿涤1. 生物安全三级实验室核心区排风高效空气过滤器原位消毒的研究[J]. 微生物与感染
, 2012, 7(3): 146-151.
HAN Wen-Dong1; SUN Zhi-Ping1; DING Yue-Na1; CAO Ming-Bo2; QU Di1. Study on sterilization of exhaust HEPA filter in situ of main area within BSL-3 laboratory. Journal of Microbes and Infections, 2012, 7(3): 146-151.
