Effect of disinfectants on killing Mycobacterium tuberculosis during surface decontamination in a biosafety level 3 laboratory
HAN Wen-Dong, SUN Zhi-Ping, DING Yue-Na, QU Di
Biosafety Level 3 Laboratory, Key laboratory of Medical Molecular Virology, Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Abstract：The application of effective disinfectants during experimental activities of highpathogenic microorganism is crucial for decontamination of laboratory environment to prevent laboratoryacquired infections. In this study, the quantitative germicidal disinfectant validation experiments using membrane filtration suspension were conducted against Mycobacterium tuberculosis (M. tuberculosis) H37Rv. Sodium hypochlorite solution (containing 1 000 mg/L active chlorine), 75% ethanol/water, and Safespore disinfectant killed M. tuberculosis efficiently. When the concentration of ethanol decreased to 37.5%, it was still able to kill M. tuberculosis by prolonging killing time to 10 min. The results suggest that 75% ethanol/water is suitable as conventional disinfectant and sodium hypochlorite solution (containing 1 000 mg/L active chlorine) is suitable under emergency and accident in biosafety level 3 laboratories (BSL3)．
韩文东，孙志平，丁悦娜，瞿涤. 结核分枝杆菌生物安全三级实验室表面去污染消毒剂杀菌效果的研究[J]. 微生物与感染, 2015, 10(5): 301-307.
HAN Wen-Dong, SUN Zhi-Ping, DING Yue-Na, QU Di. Effect of disinfectants on killing Mycobacterium tuberculosis during surface decontamination in a biosafety level 3 laboratory. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(5): 301-307.
