为探讨乙型肝炎病毒表面抗原(HBsAg)T131N/M133T变异株的糖基化和抗原性，本研究构建了T131N/M133T变异的HBsAg过表达质粒，将质粒转染细胞，用蛋白免疫印迹法检测HBsAg表达和糖基化情况，用酶联免疫吸附试验(ELISA)和免疫荧光法检测HBsAg的抗原性。结果显示，T131N/M133T变异使HBsAg产生了新的N－糖基化修饰，该变异质粒转染细胞内HBsAg水平显著低于野生型，上清液中HBsAg水平也有一定程度降低。结果提示，T131N/M133T变异使HBsAg发生了新的N－糖基化修饰，该变异产生的糖基化可能影响HBsAg的胞内稳定性，对HBsAg的抗原性也有一定影响。

To study the impact of T131N/M133T substitutions in hepatitis B virus surface antigen (HBsAg), the proteins expressed in Huh7 and HeLa cells were subjected to Western blotting, enzyme－linked immunosorbent assay (ELISA) and immunofluorescence (IF) staining. The results showed that T131N/M133T substitutions created a new site for N－glycosylation. The detected level of T131N/M133T was significantly low, indicating a potential negative impact on stability and antigenicity of HBsAg.