Effects of T131N/M133T substitutions in hepatitis B virus surface antigen on glycosylation and antigenicity
KANG Yao-yue1, GUO Hong-ying2, ZHANG -Yongmei1, ZHANG -Jiming1
1. Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, 200040, China; 2. Shanghai Public Health Clinic Center, Shanghai 200083, China
Abstract:To study the impact of T131N/M133T substitutions in hepatitis B virus surface antigen (HBsAg), the proteins expressed in Huh7 and HeLa cells were subjected to Western blotting, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence (IF) staining. The results showed that T131N/M133T substitutions created a new site for N-glycosylation. The detected level of T131N/M133T was significantly low, indicating a potential negative impact on stability and antigenicity of HBsAg.
康耀月1,郭红英2,张咏梅1,张继明1. 乙型肝炎病毒表面抗原T131N/M133T变异株糖基化和抗原性研究[J]. 微生物与感染, 2015, 10(2): 82-86.
KANG Yao-yue1, GUO Hong-ying2, ZHANG -Yongmei1, ZHANG -Jiming1. Effects of T131N/M133T substitutions in hepatitis B virus surface antigen on glycosylation and antigenicity. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(2): 82-86.