微生物与感染
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微生物与感染  2019, Vol. 14 Issue (3): 185-192    DOI: 10.3969/j.issn.1673-6184.2019.07.008
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数字聚合酶链反应及其在感染性疾病中的应用
赵云1,王蕾2,郭雅萌2,陈力2,汤慧3
1. 重庆大学生命科学学院,重庆 405200; 2. 复旦大学上海医学院基础医学院教育部、卫健委、医科院医学分子病毒学重点实验室,上海 200032; 3. 教育部感染性疾病分子生物学重点实验室,重庆医科大学病毒性肝炎研究所,重庆医科大学附属第二医院感染科,重庆 400010
Principle of digital polymerase chain reaction and its application in infectious diseases
ZHAO Yun1, WANG Lei2, GUO Yameng2, CHEN Li2, TANG Hui3
1. School of Life Sciences, Chongqing University, Chongqing 405200, China; 2. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College Fudan University, Shanghai 200032, China; 3. Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
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摘要: 数字聚合酶链反应(polymerase chain reaction,PCR)采用与定量PCR相同的荧光化学原理和不同的数学原理来实现对靶标核酸序列的绝对定量,其摒弃了对外部参照的依赖,同时具有更高的数据精密度,提高了重复性和再现性。数字PCR的应用涵盖生命科学众多领域,特别是在医学检验领域,其对疾病相关核酸分子标记的准确分析,为疾病的早期诊断、进展监测、疗效评估提供了动态量化指标。数字PCR的出现将推动基于核酸扩增技术的分子生物学检测迈入精准定量阶段。本文就数字PCR尤其是微滴式数字PCR在感染性疾病中的应用进展及前沿进行综述。
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赵云1
王蕾2
郭雅萌2
陈力2
汤慧3
关键词 数字聚合酶链反应微滴式数字聚合酶链反应泊松分布绝对定量感染性疾病    
Abstract:Digital polymerase chain reaction (PCR) is an emerging technology for quantitative analysis of nucleic acids, employing the same fluorescence chemistry as quantitative PCR (qPCR) but different mathematical principles to achieve absolute quantification of target nucleic acid sequences, without the reliance on external references such as standard curve. This technology is both conceptually simple and extremely robust in terms of assay performance, including increased sensitivity, precision, reproducibility and repeatability. Digital PCR technology has been widely used in life science research and applications. In the field of medical testing, digital PCR provides accurate quantification of disease-related nucleic acid biomarkers, enabling early diagnosis of diseases, monitoring of disease progression and evaluation of therapeutic efficacy as well. The emergence of digital PCR will push the medical testing into the stage of precise quantification. Here we review the progress and cutting edge of digital PCR technology, especially droplet digital PCR technology in the field of infectious diseases.
Key wordsDigital polymerase chain reaction    Droplet digital polymerase chain reaction    Poisson distribution    Absolute quantification    Infectious disease
通讯作者: 汤慧   
引用本文:   
赵云1,王蕾2,郭雅萌2,陈力2,汤慧3. 数字聚合酶链反应及其在感染性疾病中的应用[J]. 微生物与感染, 2019, 14(3): 185-192.
ZHAO Yun1, WANG Lei2, GUO Yameng2, CHEN Li2, TANG Hui3. Principle of digital polymerase chain reaction and its application in infectious diseases. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(3): 185-192.
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