Abstract：The present paper aimed to inhibit the expression of 3D polymerase of coxsackievirus B by short hairpin RNAs (shRNAs) with lentiviral vector. Three pairs of RNA interference sequences targeting 3D gene and corresponding control sequences were designed and synthesized. The pair with the highest interference efficiency was screened by quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting. The recombinant plasmid pLVTHM-3DshRNA was constructed and co-transfected with psPAX2 and pMD2.G in 293T cells. The packaged lentivirus (titer 5×107 TU) was subjected to functional evaluations in HeLa cells and mice. The inhibition of 3D polymerase expression was observed.
闫碧莹，徐维祯，钟照华. 靶向3D基因shRNA慢病毒载体的构建及功能鉴定[J]. 微生物与感染, 2019, 14(2): 82-88.
YAN Biying, XU Weizhen, ZHONG Zhaohua. Construction and functional verification of a shRNA targeting 3D polymerase of coxsackievirus B. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(2): 82-88.
2019, Vol. 14 
