Preparation of coxsackievirus B3 3D protein polyclonal antibody
WANG Yu1, XU Weizhen1, MU Yusong1, WANG Tianying1, YAN Biying1, YAN Xueqin1, ZHAO Wenran2, ZHONG Zhaohua1
1. Department of Microbiology and Wu Lien-Teh Institute, Harbin Medical University, Harbin 150081, China; 2. Department of Cytobiology, Harbin Medical University, Harbin 150081, China
Abstract：Coxsackievirus B3 (CVB3) mainly infects the heart, and the timing and distribution of viral RNA polymerase (3D protein) expression in the myocardium are currently unclear. In this study, the CVB3 3D gene was obtained by polymerase chain reaction (PCR) and cloned into the prokaryotic expression vector pET28a(＋). The expression of 3D was induced by isopropyl β-D-1-thiogalactopyranoside (IPTG), and the 3D-His protein was purified and used to immunize New Zealand white rabbits for antiserum against the 3D protein. An anti-CVB3 3D antibody with high titer and specificity was obtained, and it can be used for future functional study of CVB3 3D protein.
王玉1，徐维祯1，穆宇松1，王天楹1，闫碧莹1，颜学勤1，赵文然2，钟照华1. 柯萨奇病毒B3型3D蛋白抗体的制备[J]. 微生物与感染, 2017, 12(3): 172-178.
WANG Yu1, XU Weizhen1, MU Yusong1, WANG Tianying1, YAN Biying1, YAN Xueqin1, ZHAO Wenran2, ZHONG Zhaohua1. Preparation of coxsackievirus B3 3D protein polyclonal antibody. JOURNAL OF MICROBES AND INFECTIONS, 2017, 12(3): 172-178.
2017, Vol. 12 
