Detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus with enzyme-linked immunosorbent assay-based endpoint assessment
LIU Dandan1, JIAO Yongjun2, ZHANG Qianyun1, LI Zhifeng2, QI Xian2, SONG Yongchun1
1. School of Life Science, Nanjing University, Nanjing 210093, China; 2. Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China
Abstract：An enzyme-linked immunosorbent assay (ELISA) method was developed for the detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus (SFTSV). Vero-E6 cells in a 96-well micro-plates were challenged with SFTSV first, and then subjected to the treatment with an anti-nucleocapsid protein (SFTSV-NP) monoclonal antibody at different concentrations. The 50% tissue culture infective dose (TCID50) was calculated with Reed-Muench method. 10 double serum samples collected at two different stages of infection were subjected to the assay. 8 specimens demonstrated higher (>4 times) antibody levels in the recovery phase than that in the acute phase. In conclusion, the established ELISA method is specific, operation friendly and with an application potential for clinical research.
刘丹丹1，焦永军2，章倩云1，李志锋2，祁贤2，宋勇春1 . 发热伴血小板减少综合征病毒中和抗体酶联免疫吸附试验方法的建立及应用[J]. 微生物与感染, 2016, 11(3): 171-175.
LIU Dandan1, JIAO Yongjun2, ZHANG Qianyun1, LI Zhifeng2, QI Xian2, SONG Yongchun1. Detection of neutralizing antibodies against severe fever with thrombocytopenia syndrome virus with enzyme-linked immunosorbent assay-based endpoint assessment. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(3): 171-175.
2016, Vol. 11 
