Abstract：Nucleocapsid (N) protein of virus plays special roles in stabilization of viral genome, regulation of viral replication and adapting cellular circumstance. Murine hepatitis virus (MHV), the prototype of Betacoronavirus, is a classic model for the exploration of coronavirus N protein functions. Viral N proteins from detergent-treated virions and recombinant N proteins expressed in prokaryotic cells were prepared and used to immunize BALB/c mice for the preparation of polyclonal and monoclonal antibodies respectively. Antibodies with high sensitivity and specificity against the antigens were selected and subjected an assay for antigenic determinants. The results indicated that both polyclonal antiserum and mAb 2E6 recognized a region covering 58 residues motif. An enzyme-linked immunosorbent assay (ELISA) with mAb 2E6 was established for N proteins in medium supernatants and cellular lysates. The signal could be detected at 4 h after infection and the readings were consistent to viral titers.
郭佳慧，文荣，王玉燕，张浩旸，刘红，徐娅佳，叶荣. 鼠冠状病毒核衣壳蛋白的抗体制备与抗原决定簇分析[J]. 微生物与感染, 2017, 12(2): 79-88.
GUO Jiahui, WEN Rong, WANG Yuyan, ZHANG Haoyang, LIU Hong, XU Yajia, YE Rong. Preparation and characterization of antibodies against murine coronavirus nucleocapsid protein. JOURNAL OF MICROBES AND INFECTIONS, 2017, 12(2): 79-88.
2017, Vol. 12 
