Abstract:Eukaryotic expression vector containing different proteins (C, M, E and NS1-5) of dengue virus serotype 2 (DENV-2) were constructed and intramuscularly injected directly to mice for developing antibodies against DENV-2. Two weeks after the fourth immunization, sera were collected and the immune effects were evaluated by enzymelinked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA) and Western blotting. Specific antibodies could be obtained by DNA immunization, and the titers of these antibodies fluctuated between 1:400 - 1:16 127. Of those, the antibody against protein E showed the highest titer at 1:16 127 whereas the antibodies against proteins NS3, NS4b and NS5 had the lowest titer at 1:400. The results showed that all antibodies acquired by DNA immunization could produce specific immunofluorescent reactions when tested using DENV-2-infected Vero cells, suggesting that antibodies prepared by DNA immunization could specifically identify naturally infected DENV-2. Antibodies against proteins E, NS1, NS4b and NS5 could also identify heatdenatured proteins. The results generated from this study provided the base for further research.