Identification and antifungal susceptibility analysis of 1386 strains of yeast in Nanjing
LI Hui- ming1; MEI Ya-Ning2
1. Department of diagnosis，First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China；2. Department of Clinical Microbiology，First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China
Abstract：The present paper ai ms to study clinical yeast isolates and analyze their susceptibility to antifungal drugs . API 20C AUXidentification systemand CHROMagar Candida mediumwere used to i dentify a total of 1 386strains of yeast collectedin our hospital fromJanuary to December , 2008 . The Rosco disk diffusion method was used to test the susceptibility of yeast to amphotericin B, fluconazole , itraconazole , nystatin , voriconazole , 5-flucytosine , and clotri mazole . The data were analyzed by Whonet 5 .4 software . Of the 1 386 strains collected, Candida albicans ( C. albicans) was the most frequent ( 58 .95%) , f oll owed by C. t r opicalis ( 15 .95 %) , C. glabr at a ( 15 .15 %) , C. kr usei ( 2.74%) , and others ( 7.21%) . Antifungal susceptibility testing results showed that the sensitive rates of C. l usitaniae , C. par apsilosi s , C. al bicans ,
C. t r opicalis , C. glabr at a , and C. kr usei to fluconazole were 95 .45 %, 94 .29 %, 82.99%, 79.19 %, 68 .57 % and 28 .95 %, respectively . The sensitive rates of C. albicans to 5-flucytosine , amphotericin B, nystatin, voriconazole , fluconazole , itraconazole , and clotri mazole were 93 .76%, 93 .15 %, 84.58 %, 84 .09 %, 82.99 %, 80 .05 % and 78.09 %, respectively . These results suggest that the major pathogen causing deep fungal infection in our hospital is C. al bicans , f ollowed by C. tr opicalis and C. glabr ata . C. al bicans was most susceptible to the antifungal eff ects of 5- flucytosine and amphotericin B. All of the yeast isolates collected, except f or C. kr usei , were highly sensitive to fluconazole .
李汇明1; 梅亚宁2 . 南京地区1386株临床分离酵母的鉴定及药敏分析[J]. 微生物与感染
, 2010, 5(4): 214-217.
LI Hui- ming1; MEI Ya-Ning2. Identification and antifungal susceptibility analysis of 1386 strains of yeast in Nanjing. Journal of Microbes and Infections, 2010, 5(4): 214-217.
