Abstract：To investigate Clostridium difficile (C. difficile)associated diarrhea (CDAD) in human immunodeficiency virus (HIV)infected inpatients in Shanghai, C. difficile and general intestinal tract pathogenic bacteria were isolated by selective medium from abnormal fecal specimens collected from 250 HIV infected inpatients from March 2012 to March 2013. The susceptibilities of C. difficile to metronidazole, vancomycin, clindamycin and moxifloxacin were determined by E-test method. Molecular typing was performed by multilocus sequence typing (MLST). The genes tcdA and tcdB of toxins A and B were detected by conventional polymerase chain reaction (PCR). 29 clinical strains of C. difficile were isolated from 250 fecal specimens (11.6%), in which 17 strains were tcdA＋/tcdB＋ and 12 strains were tcdA－/tcdB－. All strains were susceptible to metronidazole and vancomycin. Resistance to clindamycin and moxifloxacin was found in 69.0% and 31.0% strains respectively. A total of 12 different sequence types (ST2, 3, 7, 8, 26, 35, 37, 38, 39, 54, 81, and 124) were identified. The most prevalent ST types were ST54 (6/29, 20.7%) and ST39 (5/29, 17.2%). The results suggest that the rate of positive C. difficile detection is high and should be monitored strictly to prevent outbreak.
金鑫1，钱雪琴1，胡绿荫1，李昕1，沈芳1，2. 上海地区住院人类免疫缺陷病毒感染患者中艰难梭菌的检测及其意义[J]. 微生物与感染, 2015, 10(5): 282-287.
JIN Xin1, QIAN Xue--Qin1, HU Lu--Yin1, LI Xin1, SHEN Fang1，2. Clinical characterization of Clostridium difficile-associated diarrhea in human immunodeficiency virusinfected inpatients in Shanghai. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(5): 282-287.
