Microsporidiosis incidences of human immunodeficiency virus-positive patients in Chongqing area
BAO Jialing1，2, MO Biying1，2, LI Tongxin3, LIU Min3, LI Tian1，2, PAN Guoqing1，2, ZHOU Zeyang1，2
1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715; 2. Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715; 3. Chongqing public health medical center, Chongqing 400036, China
Abstract：Microsporidia infects immunocompromised populations such as human immunodeficiency virus(HIV)-positive patients, causing diarrhea and other life-threatening symptoms. Few studies on this infection have been conducted in China. This study collected 22 HIV-positive patients in Chongqing, who have not received antiviral treatment. The immune profile analysis of these 22 patients showed the average lymphocytes were 0.93±0.13×109/L, CD4＋T cells were 132±22 cells/mL, CD8＋T cells were 495±91 cells/mL, and CD4/CD8 ratio was 0.37±0.1, indicating all the patients were immunocompromised, thus vulnerable to opportunistic infections. The total DNA was extracted from the respective stool samples. The existence of microsporidia was confirmed by PCR and DNA sequencing. The results showed that the infection rate was 36.3% (8/22), and caused by Encephalitozoon spp, mainly E. hellem and E. intestinalis. Further comparison between Encephalitozoon spp infected and un-infected patients showed that, lymphocytes were 0.51±0.1 vs 1.17±0.17×109/L, P<0.05; CD4+T cells were 71±27 vs 167±28 cells/ mL, P<0.05; CD8＋T cells were 209±35 vs 658±123 cells/ mL, P<0.05, suggesting the infected patients were severely immune-suppressed. The current study is the first investigation of microsporidia infection in HIV-positive patients in Chongqing area. It demonstrates the correlation between immune-suppression and microsporidia infection, and the high infection rate of Encephalitozoon spp in Chongqing area. Together, this provides evidence to elucidate the mechanisms of microsporidia-host interactions, as well as insights into public health control.
包佳玲1，2，莫碧莹1，2，李同心3，刘敏3，李田1，2，潘国庆1，2，周泽扬1，2. 重庆地区人类免疫缺陷病毒携带者的微孢子虫感染情况调查[J]. 微生物与感染, 2020, 15(5): 271-277.
BAO Jialing1，2, MO Biying1，2, LI Tongxin3, LIU Min3, LI Tian1，2, PAN Guoqing1，2, ZHOU Zeyang1，2. Microsporidiosis incidences of human immunodeficiency virus-positive patients in Chongqing area. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(5): 271-277.
2020, Vol. 15 
