微生物与感染
2024年11月29日 星期五   首页 |  主编心语 |  期刊介绍 |  编委阵容 |  投稿指南 |  出版道德 |  期刊订阅 |  联系我们 | English
微生物与感染  2022, Vol. 17 Issue (6): 374-379    DOI: 10.3969/j.issn.1673-6184.2022.06.005
  病例分析 本期目录 | 过刊浏览 | 高级检索 |
气管切开患者痰培养分离肢体凯斯特菌1株报道
闵彦1,武静1,李继霞1,公衍文2,胡成进1
1. 解放军第960医院检验科,山东 济南 250031;  2. 山东大学第二附属医院检验医学中心,山东 济南 250033
Isolation of Kerstersia gyiorum from patients with tracheotomy by sputum culture
MIN Yan1, WU Jing1, LI Jixia1, GONG Yanwen2,HU Chengjin1
1. Department of Laboratory Medicine, the 960th Hospital of PLA, Jinan 250031, Shandong Province, China; 2. Department of Clinical Laboratory, the Second Hospital of Shandong University, Jinan 250033, Shandong Province, China
全文: PDF (1164 KB)   HTML (141)
输出: BibTeX | EndNote (RIS)      
摘要: 本实验室在1例气管切开患者的痰培养标本中,发现了1种膜状生长的革兰阴性杆菌。使用全自动细菌鉴定仪Vitek2 Compact鉴定该细菌,没有得到阳性结果。分别使用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS)和 16S 核糖体RNA(16S ribosomal RNA,16S rRNA)测序的方法对该菌进行鉴定,结果显示为肢体凯斯特菌。该菌是一种革兰氏阴性非发酵菌。回顾国外文献仅有少量病例报道,该菌可能为条件致病菌,可在气管切开患者的痰液、肺泡灌洗液以及经皮造瘘患者的尿液中分离,还可引起菌血症并参与慢性耳炎的发生发展。国内文献对肢体凯斯特菌的分离研究罕见报道。本实验室将此病例及该菌的基本特性报道如下,以加强临床微生物检验人员对其的认识和重视。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
闵彦1
武静1
李继霞1
公衍文2
胡成进1
关键词 肢体凯斯特菌气管切开基质辅助激光解吸电离飞行时间质谱16S核糖体RNA测序    
Abstract:In the sputum culture of a patient with tracheotomy, we found a kind of Gram-negative bacteria with membrane growth, which was not identified by Vitek2 Compact. Then, the strain was identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and 16S ribosomal RNA (16S rRNA) sequencing, and the results showed that the strain was Kerstersia gyiorum. Kerstersia gyiorum is a kind of Gram-negative non-fermenting bacterium. A few of foreign cases reported that this bacterium might be a conditional pathogen, which could be isolated from sputum and alveolar lavage fluid of tracheotomy patients, and from urine of percutaneous fistula patients. This bacterium could also cause bacteremia and participate in the occurrence and development of chronic otitis media. Chinese literature on the isolation of Kerstersia gyiorum is rare. The strain of the bacterium was isolated, and the basic characteristics of the bacterium were reported as follows to enable clinical microbial laboratory personnel to strengthen the understanding and attention on it.
Key wordsKerstersia gyiorum    tracheotomy    MALDI-TOF-MS    16S rRNA sequencing
:  R446.5  
  R372  
  R378    
通讯作者: 公衍文,胡成进   
引用本文:   
闵彦1,武静1,李继霞1,公衍文2,胡成进1. 气管切开患者痰培养分离肢体凯斯特菌1株报道[J]. 微生物与感染, 2022, 17(6): 374-379.
MIN Yan1, WU Jing1, LI Jixia1, GONG Yanwen2,HU Chengjin1. Isolation of Kerstersia gyiorum from patients with tracheotomy by sputum culture. JOURNAL OF MICROBES AND INFECTIONS, 2022, 17(6): 374-379.
链接本文:  
http://jmi.fudan.edu.cn/CN/10.3969/j.issn.1673-6184.2022.06.005     或     http://jmi.fudan.edu.cn/CN/Y2022/V17/I6/374

版权所有 © 2008 《微生物与感染》编辑部
地址:上海市医学院路138号 邮编:200032
Tel:021-54237633 Fax:021-64434224    E-mail:jmi@fudan.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn