Application of bacterial 16S rDNA amplification and sequencing in the classification and identification of bacteria

ZHU Shi-Ying, QI Zhong-Tian

Journal of Microbes and Infections ›› 2013, Vol. 8 ›› Issue (2) : 104-109.

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Journal of Microbes and Infections ›› 2013, Vol. 8 ›› Issue (2) : 104-109.
Summary

Application of bacterial 16S rDNA amplification and sequencing in the classification and identification of bacteria

  • ZHU Shi-Ying, QI Zhong-Tian
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Abstract

Bacterial 16S rDNA amplification and sequencing is a new tool which has been widely used to identify bacterial species and perform taxonomic studies. The application of this technology for identification of uncultivable bacteria, differentiating species with high DNA sequence similarity and discovering novel bacterial genus and species are introduced in this paper. Future perspective of the method in clinical microbiology laboratories is also discussed.

 

Key words

16S rDNA / Polymerase chain reaction / DNA sequencing / Identification

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ZHU Shi-Ying, QI Zhong-Tian. Application of bacterial 16S rDNA amplification and sequencing in the classification and identification of bacteria[J]. Journal of Microbes and Infections. 2013, 8(2): 104-109
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