Non-fermentative bacteria can exist extensively in the environment. The use of antibiotics and cross-infection by the hands of medical personnel have made it become the major pathogen of nosocomial infection. The intrinsic resistance to many common antibiotics in non-fermentative bacteria, and antibiotic pressure have caused the rapid increase in multi-resistance rate. The resistance rate of Acinetobacter baumanii to carbapanems has risen to more than 50%. Some pathogens are resistant to disinfectors. Non-fermentative bacteria are primarily opportunistic pathogens. Underlying host factors are strongly associated with outcomes. Multidrug resistance greatly limits the therapeutic options for patients who are infected with this organism. Carbapenems is still a class of optional antibiotics, as well as polymyxin, tigecycline, sulbactam and its compounds. How to manage the patients with non-fermentative bacteria infection will be a challenge for clinicians in the future.