Drug resistance and molecular typing of carbapenem-resistant Klebsiella pneumonia(CRKP)isolated from a bloodstream infection was conducted to provide scientific basis for clinical practice, prevention, and control measures. Non-repetitive Klebsiella pneumoniae strains collected in a Shanghai tertiary hospital from April 2015 to March 2018 were subjected to the study. A total of 51 strains of CRKP were isolated. Antimicrobial susceptibility testing was carried out, the carbapenemase genes were detected by PCR, and the homology was analyzed by Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The analysis result showed that the CRKP strains were multi-resistant to antibiotics and bla_KPC-2 gene were detected in all strains. Producing bla_KPC-2 is a major resistant mechanism to carbapenems for Klebsiella pneumoniae. The results of PFGE typing showed there were dominant clones in this hospital, with ST11 being the main type (84.31%). The results indicated that surveillance of bacterial resistance is an effective control for hospital infection management.