Colorectal cancer (CRC) is a common digestive malignant tumor in China. A large number of studies have confirmed that intestinal flora disorders can promote the occurrence and development of CRC through a variety of mechanisms, and regulating intestinal flora can exert certain auxiliary therapeutic effects on CRC. But the treatment still has certain limitations and side effects. Studies have shown that the clinical treatment of CRC by engineered and edited intestinal bacteria through gene editing may have potential application values. In this paper, the current research progress on gene-editing for modifying intestinal bacteria in the treatment of CRC is reviewed, aiming to explore the possibility of gene-edited intestinal bacteria in the treatment of CRC in the future.