A case of bloodstream infection caused by Campylobacter jejuni was reported, and its biological characteristics and possible drug resistance genes was studied to provide scientific laboratory basis for effective clinical treatment of related diseases. The strain isolated from the blood of an elderly female was identified by conventional methods, matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOFMS), sequencing and polymerase chain reaction(PCR). Results show that the isolated strain was Campylobacter jejuni, and it was resistant to ciprofloxacin and tetracycline and sensitive to erythromycin. Three drug resistance genes gyrA, gyrB and tex(O) in Campylobacter jejuni were detected by PCR, which may be related to the results of drug sensitivity test.