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Abstract In order to explore the ability of routine laboratory identification methods to identify clinical isolates of Herbaspirillum huttiense(H. huttiense),the source of infection of H. huttiense in the blood of this patient was analyzed.Automated biochemical identification system, MALDI-TOF MS,16S rRNA gene sequencing and rpoB gene sequencing were used to identify H. huttiense, and the specimens from multiple parts of the patient were detected. The results showed that the automatic biochemical identification system misidentified H. huttiense as Burkholderia cepacia. MALDI-TOF MS and 16S rRNA gene sequencing were not able to distinguish between H. huttiense, Herbaspirillum aquaticum and Herbaspirillum camelliae,while rpoB gene sequencing could accurately identify H. huttiense. In addition to the blood, only a small amount of H. huttiense was detected in the patient's feces. The results of ERIC-PCR showed the same DNA band patterns of H. huttiense from blood and feces. MALDI-TOF MS MSP cluster analysis showed that they were located at the same node, and the Distance Level<50, with the same resistance phenotype. The blood and fecal origin of hatteriospirillum were highly homologous.This study demonstrated the performance of automated biochemical identification system, MALDI-TOF MS, 16S rRNA gene sequencing and rpoB gene sequencing for identification of H. huttiense, which confirmed that H. huttiense could infect gastrointestinal tract through contaminated food and cause sepsis in patients with postoperative chemotherapy for rectal adenocarcinoma.
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Received: 28 February 2023
Published: 01 January 2023
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