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| Development and applications of PCR-CE based method for detection of biovars of U. urealyticum |
| ZHOU Shi-hang; ZHANG Zhuo-ran |
| Dalian Red Cross Blood Center; Dalian 116001; China |
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Abstract Objective To develop a polymerase chair reaction-capillary electrophoresis(PCR-CE)based method for the detection of biovars one and two of U.urealyticum and to determine whether these two biovars of U.urealyticum are associated with the nongonococcal urethritis(NGU)in the male patients.Methods Common primers for two biovars of U.urealyticum were synthesized and used for the PCR.Then PCR products obtained for two biovars of U.urealyticum were detected using capillary electrophoresis(CE).Two biovars of U.urealyticum were detected by the above method in the urine samples from the different male populations.The sensitivity and specificity of this PCR-CE based method was further examined.Results This PCR-CE method had a sensitivity of detection at 10 copies /50 μl.This method specifically amplified biovar 1 and biovar 2 of U.urealyticum.The prevalence of U.urealyticum biovar 2 in NGU group and nonchlamydial NGU(NCNGU)group was higher than that in the control group(P<0.05)but the prevalence of U.urealyticum biovar 1 in NGU group and NCNGU group did not differ significantly from that in the control group(P>0.05).Conclusion A PCR-CE based method for the detection of two biovars of U.urealyticum has been developed,which has good sensitivity and specificity.U.urealyticum biovar 2 is associated with NGU in the male.U.urealyticum biovar 1 can not cause NGU.
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Received: 01 January 1900
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Corresponding Authors:
ZHANG Zhuo-ran
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2007, Vol. 2 
